浸提法与添加果胶酶提取栀子黄色素的比较

Comparison between Ethanol and Pectinase Appended in Extraction of Gardenia Yellow

探讨了栀子黄色素提取工艺中的浸提法及加果胶酶提取法,通过正交试验确定其相应的优化工艺条件,分析比较得出:加果胶酶法提取栀子黄色素与传统浸提法相比,提取的色素产品提取率高、色价高。其最佳提取工艺条件为:缓冲液pH值5,加酶量8mg/g,酶解温度60℃,酶解时间3h。然后根据加酶的最佳酶解条件,再在乙醇提取法的最佳提取条件下提取栀子黄色素。在此条件下色素的提取率为98%,产品色价85。

Ethanol and pectinase appended in extraction of gardenia yellow pigment were discussed, and the relevant optimum conditions were determined with orthogonal experiments. The results showed that the ultrasonic extraction excelled the appending pectinase in the yield and color value. The optimum conditions were determined as follow： the pH of buffer solution was 5, the quality of appending pectinase was 8 mg/g, and enzyme reacted 3h at 60℃, The yield and the color value of the extracted pigment achieved 98 % and 85 respectively.