拉曼光谱法鉴定水稻叶绿素缺乏突变体

Spectroscopic Study on the Chlorophyll-Deficient Rice Mutant

植物叶色变异是自然界普遍的现象,叶色突变体已广泛应用于基础研究和生产实践。利用拉曼光谱测定了水稻叶色黄化突变体叶片的叶绿素含量。研究结果显示:在拉曼光谱图中。叶绿素的特征峰(1155,1527cm-1)在突变体中强度较野生型有很大的下降,表明突变体中总的叶绿素含量较低;紫外分光光度计的测定结果表明该突变体的叶绿素含量比野生型降低,且叶绿素b的含量极低,证实了拉曼光谱所反应的信息;拉曼光谱法可以用来快速鉴定植物活体的叶绿素含量。本研究尝试利用拉曼光谱测定活体生物的叶绿素含量的可行性,为今后发展便携式、快速、准确、无损伤鉴定叶绿素含量及其他生物质含量的方法和开发相关仪器提供研究思路。

The chlorophyll content of rice yellow mutant was studied by Raman spectroscopy and UV Spectrophotometer. The Raman spectroscopy results show the characteristic pcaks （1155, 1127cm^-1） of chlorophyll are weaker in mutant than in wild type. The chlorophyll content was lower in mutant. The UV adsorption of the total chlorophyll content in mutant was lower than the wild type, and the chlorophyll b content is very low, which agrees with the results of FT-Raman spectroscopy. The study proved a feasibility of FT-Raman spectroscopy for the rapid and accurate analysis of chlorophyll content and other substance in plants.