香附药材质量相关性分析研究

Correlation studies on the quality of Rhizoma Cyperi

目的:对收集的8个产地香附药材的质量进行分析与评价。方法:采用 GC-MS 法分析鉴定香附药材挥发性成分,分析条件为:DB-1701石英毛细管色谱柱(0.25 mm×30 m,0.25 μm),程序升温,进样口温度220℃,分流比100:1,流速1 mL·min^(-1);采用 HPLC 法分析不同产地香附药材甲醇提取物的化学成分,分析条件为:Hedera ODS-2 C^(18)色谱柱(4.6 mm×250mm,5 μm),甲醇(A)-水(B)系统为流动相,梯度洗脱[0～20 min,5%A～50%A;20～30 min,50%A～60%A;30～70 min,60%A～80%A;70～80 min,80%A～100%A],流速1 mL·min^(-1),检测波长254 nm,柱温30℃。对8个不同产地香附药材的甲醇提取物化学成分特征进行相似度评价分析和聚类分析。结果:以 GC-MS 分析鉴定了香附挥发油中22个化合物;以HPLC 法建立了不同产地香附甲醇提取物化学成分的特征图谱;相似度评价与聚类分析结果表明山东产香附与安徽、海南、浙江、江苏产香附距离较近,聚为一类,而河南与河北产香附与上述产地香附距离较远。结论:综合分析表明,地道产地山东香附与安徽、浙江和海南产香附在性状特征和化学成分信息方面均表现出较好的趋同性,与江苏、广西和河南产香附具有一定的趋同性,而河北安国香附在性状及聚类分析中均与山东香附距离最远,表现出明显的趋异性。本文研究结果为我国不同产地香附药材的检定、识别和质量评价提供了客观依据。

Objective：To analyze the chemical components of Rhizoma Cyperi from different regions in China and to evaluate their qualities. Methods： GC - MS method was used to identify the components in essential oils of Rhizoma Cyperi from eight regions. The temperature programmed processes was performed on the column of DB - 1701 quartz capillary column（0.25 mm × 30 m,0. 25 μm）. Split injection was conducted with a split ratio of 100： 1. The injector temperature was 220 ℃,flow rate of N2 was 1 mL · min^-1. HPLC was used to analyze the components of methanol extract. And hierarchical cluster analysis was performed to find resemblance among Rhizoma Cyperi from different regions. The analytical method was developed on a Hedera ODS -2 Clscolumn（4.6 mm ×250 mm,5 μm） and the mobile phase was consisted of CH3OH（A） and H2O（B） using a linear gradient[0 -20 min,5%A→50% A;20 -30 min,50% A→60% A;30 - 70 min,60% A→80% A;70 - 80 min,80% A→100% A]. The solvent flow rate was 1.0 mL · min^-1 with UV absorbance detection 254 nm. The column temperature was set at 30 ℃. Results： Twenty - two principal constituents were identified in the volatile oils by GC - MS. RP - HPLC method was established for analysis of methanol extract of different resources Rhizoma Cyperi. Cluster analysis results showed that Rhizoma Cyperi from Anhui, Hainan, Zhejiang, Jiangsu, Guangxi could be gathered one group with Shandong sample based on the HPLC characteristic peaks. And samples from Henan and Hebei were most diversity with Shandong Rhizoma Cyperi. Conclusions： Rhizoma Cyperi from Anhui, Zhejiang and Hainan showed good convergence with the genuine area （Shandong） sample; while Hebei sample showed obvious divergence with Shandong sample. The chemical information of genuine area （Shandong） sample showed some degree convergence with Rhizoma Cyperi from Jiangsu, Guangxi, and Henan. The results provided objective evidences for identifying and evaluating quality of Rhizoma Cyperi from different resources.