RP-HPLC测定乙肝解毒胶囊中黄芩苷的含量

RP-HPLC determination of baicalin in antidotal capsules

目的:建立 RP-HPLC 法测定乙肝解毒胶囊中黄芩苷的含量,以有效控制该制剂黄芩苷的质量。方法:采用 VP-ODS(150 mm×4.6 mm,5 μm)色谱柱,以甲醇-水-磷酸(50:50:0.1)为流动相;检测波长:278 nm;柱温28℃;流速1.0 mL·min^(-1)。结果:黄芩苷在6.05～121.0μg·mL^(-1)浓度范围内呈良好的线性关系(r=0.9999),平均回收率为97.9%(RSD=0.95%),精密度可靠(RSD=0.7%),样品在24 h 内稳定。结论:本文建立的含量测定方法简便快捷,结果准确且重现性好,可用于乙肝解毒胶囊的质量控制。

Objective： To establish a method for the determination of baicalin in antidotal capsules. Method： Baicalin was determined by RP- HPLC with methanol -water- phosphric acid（50： 50： 0. 1 ）in chromatographic system, VP -ODS（ 150 mm ×4. 6 mm,5 μm） as the column,278 nm as the detecting wavelength,28 ℃ as the column tem- perature and the flow rate was 1.0 mL ·min^-1. Results：The linear correlation was good in the 6.05 - 121.0μg ·mL^-1 of baicalin,correlation coefficient was 0. 9999. The average recovery was 97. 9% and RSD was 0. 95%. Conclusion：The method is simple, accurate, repeatable and can be used for quality control of antidotal capsules.