食品中脱氧雪腐镰刀菌烯醇三种检测方法的比较研究

Comparasion of the detection methods for deoxynivalenol in corn

对谷物中DON的检测方法进行了研究,确定了各检测方法的参数,HPLC方法最低检出限为52μg/L,加标回收率为78.92%,相对标准偏差RSD为1.88%,精密度很高;ELISA方法的最低检出限为10μg/L,加标回收率为104%,RSD为5.8%;金标试剂条检测可做出一个标准的颜色反映系列,重现性很好,最低检出限为1000μg/L,加标回收率也很高。金标检测方法简便、快速,适用于现场检测,是未来检测方法的发展方向。

The detection methods of vomitoxin and determined the detection parameters were studied. The minimum limition of HPLC was 52μg/L,the recovery ration was 78.92% ,the RSD was 1.88% ,the precision was very high. And the parameters of ELISA were： minimum limition 10μg/L, the recovery ration 104%, RSD 5.8%. The detection result of the gold-labeled strip could be made a standard color reflected series,the repeatability is very well,minimum limition 1000μg/L,and the recovery was also high. The simple and quickgold-labeled method will be the best method in future,