适应性训练对骨骼肌肌力、骨架蛋白含量的影响

Effects of Adaptive Training on Muscle Force and Content of Cytoskeleton Protein

以雄性Wistar大鼠为研究对象,进行"3周、6周适应性离心运动训练"两种方式运动。其中3周适应性离心训练(渐进增加强度),每周5天训练,速度从20.0 m/min增加到32.5 m/min,每次20 min,-5°下坡间歇跑。6周适应性离心训练(相同强度),每周5天训练,前3周速度从20.0 m/min增加到32.5 m/min,后3周保持35.0 m/min,每次20min,-5°下坡间歇跑。离心运动后不同时段和适应性训练后取大鼠后肢腓肠肌外侧头进行分析。6周适应性训练组进行一次性大强度离心运动,一次性离心运动实验为-16°下坡跑台跑,定量大负荷间歇性运动,跑速为26.8 m/min,运动5 min×10组,组间歇1min。研究适应性训练后大鼠腓肠肌收缩力和拉断力和骨骼肌细胞骨架蛋白含量的变化特点;分析肌收缩力和拉断力与肌细胞骨架蛋白缺失的关系以及与延迟性骨骼肌损伤时血清酶升高的关系;通过观察大鼠经过适应性训练后,骨骼肌肌力及肌细胞基质蛋白含量是否会有适应性的变化,是否会因此提高对抗大强度离心运动所致骨骼肌损伤的能力。

Male wistar rats were performed 3-week and 6-week adaptive training respectively. 3- week physical training involves the 5 days/wk, and the speed from 20. 0m/rain in the first week to 32.5m/min in the last week, and the grade was always -5°, 6-week physical training involves the 5days/wk, and the speed from 20.0m/min in the first week to keep 35.0m/min in the last three weeks, and the grade was always -5°. The purpose of this study was to investigate the change of the maximum force of the skeletal muscle affected by eccentric exercise and physical training. The change of the contents of the cytoskeleton protein affected by eccentric exercise and physical training is analyzed to show the relationship among the maximum force of the skeletal muscle and the loss of the cytoskeleton protein and the change of the serum enzyme after the exercise lead to muscle injury. Through investigation, the function of the cytoskeleton protein in skeleton muscle fiber is further discussed.