高温对蚕豆幼嫩子叶细胞有丝分裂活性和染色体分带的影响

The High Temperature Affect on Mitosis Activity and Chromosome Banding of the Young Cotyledon in Vicia faba

用不同温度对蚕豆子叶进行短时间的非离体处理，结果表明３６℃的高温最适宜子叶细胞的分裂，有丝分裂指数明显高于１６～２２℃（结荚期适宜温度）时的分裂指数，二者差异非常显著．用经３６℃高温处理的子叶做材料进行染色体制片和分带，染色体分散好，没有细胞质干扰，带纹清晰，首次获得了蚕豆子叶染色体Ｃ－带核型图．但是，超高温（４０℃）抑制细胞分裂。

Young cotyledon of Vicia faba are treated under different temperature in living plant for short time. The results show the temperature 36℃ is best suitable for the cell division of cotyledon. Under this temperature,the mitosis index is higher remarkablely than the index between 16～22℃(suitable temperature for bearing bean-pod). The results using cotyledon treated under 36℃for preparing chromosome and chromosome banding show chromosome disperse better,no coytplasm interference,and band stria is clear. The research obtain the C-bands of chromosomes karyogram of cotyledon in Vicia faba firt. Over temperature 40℃ once,the cell division would be repressed,and the chromosome structures would be destroied.