输氧湿化瓶微生物污染及污染途径调查

Investigation of microbial contamination of bottle for oxygen humidification and the way of contamination

目的调查输氧湿化瓶的污染途径,了解其微生物污染情况及所检出致病菌的抗生素敏感度。方法对正在使用的输氧设施345套次的不同部位及痰和病房空气等处分别取样做细菌培养;针对致病菌铜绿假单胞菌进行药敏试验。结果湿化液严重污染占37.1%;湿化瓶和湿化液连续使用时间越长细菌检出率越高,且严重污染者亦随之升高,使用时间〉3d与〈1d对比差异显著（P〈0.05）;湿化瓶盖、鼻导管、咽拭子、痰及病房空气培养检出的细菌种类与湿化液检出的细菌种类相似。结论湿化液的常见污染菌多为条件致病菌。本研究提示湿化液的污染途径为经鼻导管的逆行污染。

Objective To investigate the way of contaminated humidification bottle of oxygen therapy and to acknowledge the condition of contaminated microorganism and master antibiotic sensitivity of the pathogenic bacteria. Methods The samples taken respectively from various parts of 345 wares of using oxygen transferring facilities, sputum and air in the hospital room were bacterial cultured. If pathogenic bacteria was Pseudomonas aeruginosa, susceptibility test was done. Results Seriously contaminated humidification fluid was 37.1%, the longer the time using humidification bottle and humidification fluid, the higher bacterial detection rate and seriously contaminated rate was. 〉3 using days and 〈1 using days are distinguished different （P 〈 0.05）. The checked bacterial sorts from bottle caps, nasal catheter, swab and air in the hospital room are similar to the checked ones from humidification fluid. Conclusions Frequently contaminative bacterium in humidification fluid is always conditional pathogenic bacteria. Regressive pollution through nasal catheter as the contaminated path of humidification fluid is warranted.