细胞角蛋白13在全反式维甲酸和三氧化二砷诱导分化人口腔鳞癌细胞系KB中的表达

Expression of cytokertin 13 in differentiation-inducing human oral squamous carcinoma cell line KB by all-trans retinoic acid and arsenic trioxide

目的研究细胞角蛋白(cytokertin,CK)13及其基因在全反式维甲酸(all-trans retinoic acid,ATRA)和三氧化二砷(arsenic trioxide,As2O3)诱导分化人口腔未分化鳞癌细胞系KB中的表达和意义。方法采用MTT法观察ATRA和As2O3对KB细胞的敏感程度,Western blot法和RT-PCR法观察KB细胞经ATRA和As2O3诱导分化后的CK13和CK13-mRNA的表达。结果MTT法显示ATRA和As2O3的IC50分别为35.9×10-6mol/L和1.55×10-6mol/L,As2O3对KB细胞药物化学敏感性比ATRA高;Western blot法显示ATRA和As2O3对KB细胞诱导分化后24h CK13无表达,48h CK13出现弱表达,72h CK13出现明显的表达;RT-PCR法显示ATRA和As2O3对KB细胞诱导分化后24h CK13-mRNA表达明显升高,48h达到高峰,72h出现下降的趋势,并且CK13-mRNA的表达变化提前于CK13的表达。结论CK13可作为口腔鳞癌诱导分化标记物和判断临床口腔鳞癌诱导分化疗效的指标。

Objective To investigate the expression of cytokertin （CK）13 and its mRNA in differentiationinducing human oral squamous carcinoma cell line KB by all-trans retinoic acid （ATRA） and arsenic trioxide （As2O3 ） and to discuss the significance of such expression in differentiation-inducing oral squamous cell carcinoma （OSCC）. Methods The sensitivity of ATRA and As2O3 on differentiation-inducing KB cell lines was detected by MTT, and the expression of CK13 and its mRNA in KB cells differentiation-induced by ATRA and As203 were examined by means of Western blot and RT-PCR. Results MTT showed that ICS0 of ATRA and As2O3 was 35.9 × 10^-6 mol/L and 1.55 × 10^-6 mol/L respectively. Western blot showed that the expression of CK13 was not found at 24 h, faint at 48 h and became intense at 72 h. RT-PCR demonstrated that the expression of CK13-mRNA increased markedly at 24 h, and reached its peak at 48 h, and then decreased at 72 h. The changes in expression of CK13-mRNA was prior to that of CK13. Conclusion CK13 might be used as a marker in differentiation-inducing oral squamous cell carcinoma and an clinical indicator in judging the curative effects.