APCP对人胃腺癌细胞HGC-27生长及血管内皮生长因子的影响

The Effect of APCP on Growth and Vascular Endothelial Growth Factor in Human Gastric Adenocarcinoma Cell HGC-27

目的体外应用CD73(5’-核酸酶)特异性抑制剂APCP(alpha,beta-methylene adenosine-5’- diphosphate,5’-α,β-亚甲基-二磷酸腺苷),探讨其对人胃腺癌细胞HGC-27细胞生长及其对血管内皮生长因子VEGF-C、VEGF-D表达的影响。方法体外培养HGC-27细胞达指数生长,设正常组和APCP不同浓度干预组,倒置显微镜观察各组细胞生长状态,MTT法检测各组细胞活性,免疫组化法观测两组细胞VEGF-C、VEGF- D的表达及其积分光密度。结果同正常组相比,随药物浓度增加,APCP各干预组细胞在生长方面表现为生长缓慢,胞浆皱缩,细胞界线模糊,细胞数量减少(P<0.05);各干预组细胞内VEGF-C、VEGF-D的表达低于正常对照组(P<0.05)。结论APCP抑制HGC-27细胞生长,细胞数量减少,降低细胞内VEGF-C、VEGF-D的表达。

Objective A specific Ecto-5′-nucleotidase （CD73） inhibitor, APCP（alpha,beta-methylene adenosine-5′- diphosphate） was used in vitro to investigate the cellular growth and expression of vascular endothelial growth factor （VEGF） in human gastric adenocarcinoma cell HGC-27. Methods HGC-27 cells were cultured for exponential growth in vitro , and devided into normal group and APCP interfered group. Inverted microscope was used to observe the cell growth state of different groups, cell activity of different groups was detected by MTr, immunohistochemistry method was used to observe the expression of VEGF-C and VEGF-D and integral optical density was determined. Results Compared to normal group, cells in APCP interfering group grew torpidly in cellular growing with endochylema shrinked, cellular terminal line changed ambiguously and number of cells decreased as the concentration of APCP increased （P 〈0.05）, expression of VEGF-C and VEGF-D in APCP interfered groups decreased （P 〈 0.05）. Conclusion APCP inhibited the growth of HGC-27, decreased the number of cells, reduced the expression of lymphangiogenesis factors VEGF-C and VEGF-D.