DMSO诱导MCF-7细胞凋亡的研究

Effect of DMSO on Apoptosis of MCF-7 Cells

目的研究二甲基亚砜(DMSO)对MCF-7细胞凋亡的诱导作用。方法用不同浓度DMSO处理体外培养的MCF-7细胞,应用倒置光显微镜观察细胞形态学变化,用MTT比色法检测细胞存活率;Hoechst33258/PI荧光染色,用荧光显微镜分析凋亡细胞比率;琼脂糖凝胶电泳检测DNA梯状条带。结果在倒置光学显微镜下观察1%DMSO处理细胞12h后细胞形态发生变化。约有50%以上的细胞变圆,细胞内有多泡小体形成。随DMSO浓度增加和作用时间的延长,细胞存活率明显下降,经MTT检测其IC_(50)值为1%;荧光显微镜下可见60%以上细胞核染色质凝集,核碎裂等凋亡细胞的形态学变化;琼脂糖凝胶电泳呈现梯状条带(DNA ladder)。结论适当浓度的DMSO能够抑制乳腺癌细胞增殖并诱导其凋亡。

Objective To investigate the effect of dimethyl sulfoxide （DMSO） on the apoptosis of MCF-7cells. Methods We added different concentrations of DMSO into the medium in which the MCF-7 cells were cultured in vitro. The morphologic changes of cells were detected by inverted phase contrast microscope. The survival rate of cells was examined by methyl-tetrazolium （MTT） assay. The apoptotic proportion was analyzed by fluorescence microscopy, DNA ladder was detected by agarose gel electrophoresis. Results The morphocytology of MCF-7 cells changed in 1% DMSO for 12h, with 50% cells rounding and multivesicular bodies formed. The cell survival rates decreased as the increase of DMSO concentration and culturing time. The IC50 value of DMSO for MCF-7 cells was 1%, with typical nuclear condensation and fragmentation under fluorescent light microscope, and ＂DNA ladder＂ appeared on a 1.5% agarose gel. Conclusion The breast cancer cell proliferation was inhibited and apoptosis was induced by proper concentrations of DMSO.