小鼠骨髓间充质干细胞的分离培养及形态学观察

The isolation、culture and morphological observation of mouse bone marrow-derived mesenchymal stem cells

目的建立一种简单实用的小鼠骨髓间充质干细胞(MSCs)的分离培养方法,通过了解其细胞生物学特性,为MSCs的应用提供实验依据。方法采用差速贴壁法体外分离、扩增MSCs,倒置显微镜观察原代及传代细胞的形态及生长过程。结果在小鼠骨髓间充质干细胞的培养过程中,血液系细胞在换液过程被去除,成纤维细胞污染经差速贴壁法也可去除。获得的骨髓间充质细胞形态较均一,生长状态良好。结论采用差速贴壁培养法可获得一定纯度的MSCs,此法简单、实用,并且获得的细胞生长状态良好,增殖能力强.

Objective Here we explore a simple and pragmatic way for culturing mouse marrow desmohemoblast stem cells （MSCs） in vitro, to provid a experimental base for applying MSCs after understanding its bionomical characteristic. Methods Different-speed adherning way was used to separate and amplify MSCs, and inverted microscope was employed to observe the proliferating process and their morpheus of primary and passage cells. Result During the process of MSCs culturing, blood cells were washed out from the medium when changed, fibroblast cells were got rid of by different-speed adherning ways. Obtained MSCs were homogeneous in cell mophous with good living condition. Conclusion Different-speed adherning culture can be used for producing a certain purity of MSCs, which is an easy way to ensure a good condition and powerful productivity for cells.