移植前供鼠口服受鼠脾细胞对脾细胞移植后GVHD的影响研究

Effect of Recipient Mouse Splenocytes Taken Orally by Donor Mouse on GVHD after Splenocyte Transplantation

本研究探讨经口服受鼠脾细胞诱导耐受的供鼠细胞输注是否能减轻移植物抗宿主病(GVHD),并保留的GVL效应。以雄性DBA-2(H-2d)小鼠为供者。供鼠分别用BALB/c鼠脾细胞、DBA-2鼠脾细胞、牛血清蛋白和普通饲料喂养,隔天1次。应用单向混合淋巴细胞反应(MLR)评估诱导耐受状态。以雌性BALB/c小鼠(H-2d)为受者,给予6.0Gy60Coγ射线全身照射,同一天输注3×103鼠白血病细胞,尾静脉输注源于DBA-2小鼠的2×107脾细胞。对照组不给予鼠白血病细胞输注。结果显示,接受已耐受供鼠脾细胞输注的受鼠发生GVHD较其他组轻,流式细胞术检测表明CD4+/CD8+比值增加。接种白血病细胞的受鼠活存率各组间无差别。结论:口服受鼠脾细胞耐受的供鼠,其脾细胞输注给受鼠后GVHD明显减轻,且对GVL效应无影响。

This study was aimed to explore whether the GVHD in mice can be ameliorated and the GVL effect in mice can be reserved by transfusion of lymphocytes of donors fed with recipient splenocytes effect. Male （ DBA-2 ） mice （ H-2d） as donors were fed with BALB/c splenocytes, DBA-2 splenocytes ,bovine serum albumin, or regular chow, every other day . Induction of tolerance was assessed by a mixed lymphocyte reaction （MLR）. Female （ BALB/c ） mice （ H- 2d ） as recipients received total body irradiation（TBI） of 6.0 Gy （^60Coγ-ray） followed by inoculation of 3 × 10^3 P388 mouse leukemia cells on the same day. Subsequently, tail vein injection of 2 × 10^7 splenocytes supplied by DBA-2 was undertaken. Control groups were fed identically without leukemia cell inoculation. The results showed that GVHD was significantly ameliorated and CD4^＋/CD8^＋ ratio increased in recipient-mice transplanted with splenocytes of tolerated dornors, compared with control animals. There was no significant difference in survival rate between different groups of recipients inoculated with leukemia cell. It is concluded that the peroral recipient-mouse splenocytes can ameliorate GVHD without hampering effect on GVL.