两种高压静电场对大鼠血细胞及血清生物化学指标的影响

Effect of two different high voltage electrostatic fields on hematology and serum biochemical parameters in rats

目的:设计两种高压静电场,并观察电场作用下大鼠外周血及一些血清生物化学指标的变化,以评价高压静电场的血液学安全性。方法:①高压静电场设计:根据高电位治疗仪的工作原理,设计两种不同类型的高压静电场,其最大电压均为26kV,而电场性质不同,分别为稳恒高压静电场和经1Hz调制的超低频脉动高压静电场。②动物实验:选取普通级成年Wistar大鼠36只,8周龄,雌雄各半,按体质量随机分为对照组A、处理组B和处理组C,每组雌雄各6只。处理组B和处理组C应用自制大鼠专用高压电场发生装置分别给予稳恒高压静电场和经1Hz调制的超低频脉动高压静电场,对照组A不经电场处理,干预12周。③观察指标:实验第6,12周取大鼠尾血测定血常规参数;实验第12周眼眶静脉丛采血测量血液生化学指标。结果:36只大鼠均进入结果分析。高压静电场暴露第6,12周时,各组大鼠外周血血常规各项指标差异均无统计学意义(P>0.05)。两个处理组的雌性大鼠丙氨酸转氨酶活性均明显低于对照组(P<0.01),其中处理组C的下降幅度更大;同时处理组C大鼠的血清钙离子浓度明显高于对照组(P<0.01),无性别差异。结论:两种新型高压静电场对机体外周静脉血血细胞的数量、形态,肝肾功能及其他代谢过程均未产生明显毒副作用;经电场处理后血清钙离子浓度增高、丙氨酸转氨酶浓度降低,则正是其生物治疗效应的体现。

AIM： To design two kinds of high voltage electrostatic field （HVEF） and then observe their effect on hematology and serum biochemical parameters in rat, so as to evaluate the hematologic safety of HVEF. METHODS： ①According to the principle of high electric potential therapeutics, two kinds of HVEF were designed. The peak voltage was 26 kV. The electrostatic fields were divided into steady HVEF and ultra-low HVEF by 1 Hz electrostatic field based on field characters. ②Thirty-six 8-week-old Wistar rats were selected （half male and half female） and randomly divided into control group A, treatment group B and treatment group C with 6 males and 6 females in each group. The two treatment groups were respectively exposed to steady HVEF and 1 Hz electrostatic field for 12 weeks. The animals in control group were not given any treatment. ③Blood parameters were measured by the tail blood of rats in the 6th and 12th weeks. Blood biochemical indexes were determined by blood from orbital veinous plexus in the 12th week. RESULTS： Thirty-six rats were all included in final analysis. Between the two treated groups and the control group, there was no significant difference in blood physiological parameters in the 6th and 12th weeks （P〉0.05）. Alanine transaminase in female rats of two treatment groups was significantly lower than that in control group （P〈0.01）, especially treatment group C. In addition, the serum Ca^2＋ concentration of 1 Hz electrostatic field was remarkably higher than that in control group （P〈0.01）, but there was no sexual difference. CONCLUSION： The two kinds of high voltage electrostatic field have little toxicity on the number and configuration of peripheral blood cells, hepatic or renal function. Furthermore, the electrostatic field can increase serum Ca^2＋ concentration and decrease serum alanine transaminase concentration, which is just its biomedical cure effect.