胎肝来源肝母细胞的制备方法

Preparation of hepatoblasts from fetal liver tissues

学术背景:在重症肝病等待肝移植过程中,应用肝母细胞治疗是可即时实施的方法之一。从胎肝中高效率的分离纯化优质细胞来源的肝母细胞具有重要的现实意义。目的:介绍制备胎肝来源的肝母细胞所涉及到的操作方法,并将这些方法横向对比,进而指出它们各自的优势与缺点,为今后实验和临床根据不同需要选择制备肝母细胞的具体方法提供依据。检索策略:应用计算机检索PUBMED数据库1997-01/2007-08有关从胎肝中分离纯化肝母细胞的文献,检索词为"fetal liver progenitor/hepatoblasts,isolation/purification",限定文章语言种类为English。同时检索CNKI数据库1997-01/2007-08有关胎肝细胞分离纯化的文献,检索词为"肝,胚胎,上皮前体细胞/肝母细胞",并限定文章语言种类为中文。另外于吉林大学图书馆查阅相应书籍及部分外文。对资料进行初审,对发表于近5年内权威杂志的文章优先考虑。排除重复研究或Meta分析类文章。共收集到245篇文献,选出其中具有代表性的介绍肝母细胞离散及分离纯化方法的文献33篇。文献评价:31篇文献中10篇文献用以介绍研究背景;5篇文献研究了胎肝细胞离散的问题;9篇文献研究了肝母细胞分离纯化的问题;7篇文献指出了该领域存在的问题及发展前景。资料综合:这些从胎肝中分离纯化出具有肝细胞和胆管上皮细胞双向分化潜能的肝上皮细胞前体细胞(又称肝母细胞),能在体外增殖并诱导分化,移植体内后显示出良好的归巢性、整合能力和再生修复功能。说明从胎肝中分离纯化肝母细胞的技术日臻完善,该领域的研究成果将有力推动以细胞为基础的肝病治疗方案在临床中的应用。结论:对各种分离纯化方法的改进和组合应用,以及新的高特异性抗原的发现有助于胎肝来源肝母细胞纯化效率的提高。

BACKGROUND： Hepatoblasts are one of high-grade cell sources for severe liver disease treatment. Efficient separation and purification of hepatoblasts from fetal liver has important practical significances. OBJECTIVE： To introduce the preparation of hepatoblasts from fetal liver, conduct a transversal comparison of these preparation methods, and point out their advantages and shortcomings, so as to provide the evidences for the experimental and clinical choices of hepatoblasts. RETRIEVAL STRATEGY： Using the key words of ＂fetal liver progenitor/hepatoblasts, isolation/purification＂, we searched the articles about fetal liver hepatoblasts separation and purification in PUBMED between January 1997 and August 2007 in English. At the same time, we searched the relative articles in CNKI using the key words of ＂liver, embryo, endothelial progenitor/hepatoblasts＂ between January 1997 and August 2007 in Chinese. In addition, some related books and foreign articles were checked in the laboratory of Jilin University. After the first examination, the articles published in the authority magazines within five years were given prior consideration. Exclusive criteria were those repetitive researches or Meta-analysis. Totally 245 articles were collected, we selected 33 representative articles about hepatoblasts separation and purification methods. LITERATURE EVALUATION： Among 31 included articles, 10 ones introduced the study background, 5 ones studied the fetal liver cell dissociation, 9 ones referred to the hepatoblasts separation and purification, and 7 ones indicated the problems and prospect in this field. DATA SYNTHESIS： The hepatic epithelial cell precursor cells （also known as hepatoblasts） with hepatic and biliary epithelial cell differentiation potential isolated from fetal liver, could in vitro proliferate and differentiate, after transplantation in vivo they show a good homing, and the ability to integrate and regenerate. Hepatoblasts isolation technology is being improved daily, and the related researches on this field will boost cell-based liver therapy in clinical application. CONCLUSION： Improvements and combination of the various isolation methods, as well as new and high-specific antigen discovery will further improve the isolation efficiency of hepatoblasts from fetal liver.