三倍频Nd:YAG激光器不同扫描速度对猪脱细胞真皮基质微孔孔径的影响

Effect of Scanning Speed of Frequency-Tripled Nd:YAG Laser on Aperture Size of Micropores in Porcine Acellular Dermal Matrix

目的探讨三倍频Nd∶YAG激光器不同扫描速度对猪脱细胞真皮基质微孔孔径的影响。方法在输出功率、频率等参数固定的前提下,调整激光束扫描速度（10-600mm/s）,以轮廓迂回法对猪脱细胞真皮基质进行打孔。分别测量打孔后真皮基质TritonX-100溶液浸泡洗涤前、后微孔入口及出口的孔径,计算真皮基质热损伤率。结果激光束扫描速度小于或等于80mm/s,热损伤率较低,微孔孔径变化不明显;随着激光束扫描速度的加快,热损伤率增大,孔径随之增大;当扫描速度加快至500mm/s,孔径开始缩小;当扫描速度为600mm/s,激光束不能穿透真皮基质形成微孔。结论不同激光束扫描速度下,真皮基质微孔形成情况不同,综合考虑热损伤率与打孔效率,80mm/s为最适宜的激光束扫描速度。

Objective To investigate the effect of scanning speed of frequency-tripled Nd ： YAG laser on aperture size of microperes in porcine acellnlardermal matrix（ PADM ）. Methods With output power and frequency constant, scanning speed of frequency-tripled Nd ： YAG laser varied from 10 mm/s to 600 mm/s. Micropores were produced on porcine acellnlar dermal matrix （PADM） with frequency-tripled Nd ： YAG laser by circuitous method. The diameter of inlet and outlet of micropores was measured, and thermal damage rate of PADM was calculated. Results When scanning speed of frequency-tripled Nd ： YAG laser was no more than 80 mm/s, the thermal damage rate was lower, and the aperture size of microperes varied indistinctively. The thermal damage rate and aperture size increased as scanning speed varied from 1130 mm/s to 400 mm/s. However, the aperture size decreased as scanning speed was up to 500 mm/s. Laser could not penetrated PADM when scanning speed reached 600 mm/s. Conclusions The aperture size of micropores in PADM varies with scanning speed of frequency-tripled Nd ： YAG laser. In view of the thermal damage rate and punching efficiency, 80 mm/s is considered the optimal scanning speed.