高效液相色谱法测定槟榔花粉中的芸苔素内酯

Determination of Brassinolide in Areca catechu Pollen by HPLC

采用高效液相色谱(HPLC)法测定槟榔花粉中芸苔素内酯的含量。使用C18柱,以乙腈:水为流动相,采用荧光检测器对样品中的芸苔素内酯进行测定,外标法进行定性定量分析。结果表明,该方法快速、灵敏、可靠、具有简便、重现性好的特点。

HPLC was used to determine the content of brassinolide in Areca catechu pollen. With acetonitrile-water as mobile phase and C18 columm used, the brassinolide in samples were determined by fluorescence detector and the qualitative and quantitative analyses were conducted by external standard method. The results showed that the method was fast, sensitive and reliable and had characters of convenience and good repeatability.