水稻不同外植体组织培养的差异性及其后代变异的研究

Study on the Difference of Tissue Culture with Different Explants in Rice and Their Offspring Variations

[目的]选用适宜的外植体,使水稻出苗率最高且最易获取有利变异。[方法]以成熟胚、幼胚、幼穗及花药为外植体进行水稻组织培养,考察同种培养条件下4种外植体的诱愈率及诱苗率,并调查各种方法所获后代的有利变异率。[结果]以成熟胚为外植体时诱愈率最高;以花药为外植体时获得的愈伤组织诱苗率最高;以成熟胚为外植体时获得的后代材料有利变异率最高。[结论]该研究为水稻育种提供了参考依据。

[Objective] The research aimed to select suitable explants to make the seedling emergence rate of rice highest and obtain the favorable variations most easily. [Method] Mature embryo, immature embryo, immature ear and anther were taken as explants to make tissue culture of rice. The callus induction rate and the seedling induction rate of 4 kinds of explants under the same culture conditions were investigated and the rate of favorable variations in the offspring obtained by all kinds of methods was surveyed. [Result] The callus induction rate was highest when mature embryo was taken as explant. Tne seedling induction rate was highest when anther was taken as explant. The rate of favorable variations was highest in the offspring obtained by taking mature embryo as explant. [Conclusion] The research provided reference basis for the breeding of rice.