摘要
目的检测cripto基因mRNA在结直肠癌及其癌旁组织中mRNA表达情况，并探讨其在结直肠癌发生发展中的意义。方法提取36例人大肠癌及癌旁正常组织中总mRNA，采用实时荧光定量PCR法检测其中cripto mRNA表达情况；并分析其表达与患者淋巴结转移、肿瘤分化程度和Dukes’s分期相关性。结果cripto mRNA表达在大肠癌组织中阳性表达率为86．1％（31／36），而正常肠组织未见cripto mRNA表达，统计学差异显著（P〈0．0001）。criptomRNA表达与浆膜侵袭、淋巴结转移和Duke’s分期密切相关（P〈0．05，P〈0．05，P〉0．05），而与患者肿瘤发生部位和分化程度无关（P〉0．05，P〉0．05）。结论cripto基因在结直肠癌组织中高表达，可能与大肠侵袭痈转移有关。
Objective To investigate the expression and significance of cripto in human colorectal carcinoma. Methods The expression of cripto mRNA in 36 samples of colon cancer tissues and adjacent normal tissues were determined by real time RT-PCR assay,and 2^-△△C1≥2 as a positive standard. Results The positive rate of cripto expression in colorectal carcinoma tissue is 86. 1% , however,tumor adjacent tissue is 0. Cripto mRNA was related with serosa invasion,lymph node metastasis and Duke＇s stage, but not with tumor location, and pathological grading. Conclusion Cripto is closely correlated to colorectal carcinoma progression, and cripto might he a potential marker for metastasis of colon cancer progression.
出处
《中国临床实用医学》
2008年第2期12-14,共3页
China Clinical Practical Medicine
基金
镇江市社会发展基金资助项目（项目编号：SH2006019）：广东省佛山市卫生局资助项目（项目编号：2004171）
