奶牛源性双歧杆菌的分离及耐药特性

Isolations of the Bifidobacterium from cows and their resistance characteristics to given antibacterial drugs

为探究奶牛源性双歧杆菌的耐药特性,用双歧杆菌选择性培养基对当地25头乳牛粪便进行了双歧杆菌的分离,筛选耐特定抗菌药菌株,对其进行属种鉴定,选择鉴定为双歧杆菌的7株菌进行12种抗菌药耐药特性研究。结果表明,试验双歧杆菌菌株(以下称试验菌株)中2株被鉴定为豚双歧杆菌(Bifidobacterium choleyium),4株为链状双歧杆菌(Bif. catenulatum),1株为青春双歧杆菌(Bif. adoleacentis)。在抗菌药浓度一定的条件下,试验菌株对四环素(Tetracycline Hydrochloride)、氯霉素(Chloramphenicol)和红霉素(Erythromycin)敏感;对诺氟沙星(Norfloxacin)、乙酰螺旋霉素(Acetylspiramycin)、盐酸左氧氟沙星(Levofloxacin)、氨苄西林(Ampicillin)、头孢氨苄(Cephalexin),因菌株不同,敏感性不同;但对链霉素(Streptomycin)、庆大霉素(Gentamycin)、阿米卡星(Amikacin)和甲硝唑(Metronidazole Tablest)表现出强的耐受性,并且,试验菌株用三种方法均未检测到质粒。

7 strains of Bifidobacterium were isolated from the feces of 25 cows in Hohhot by selective culture medium. 2 of them are Bifidobacterium choleyium ,4 are Bifidobacteium catenulatum and 1 is Bifidobacterium adoleacentis . Their sensitivity to 12 kinds of antibacterial drugs was detected. It was indicated that 7strains were sensitive to Tetracycline Hydrochloride, Chloramphenicol and Erythromycin, and resistance to Streptomycin, Gentamycin, Amikacin and Metronidazole Tablest. But 7strains showed different sensitives to Norfloxacin, Acetylspiramycin, Levofloxacin, Ampicillin and Cephalexin. There were no plasrnids DNA by determine of them with three methods.