摘要
为了从蛋白水平研究成纤维细胞生长因子7(FGF7)及其受体(KGFR)在哺乳动物乳腺发育、泌乳及退化过程中表达定位的动态变化,揭示FGF7及其受体表达变化与乳腺发育及泌乳功能的关系,本研究利用激光扫描共聚焦显微系统对昆明鼠乳腺中FGF7及其受体的表达进行了免疫荧光检测,利用体外培养不同发育时期的小鼠乳腺组织研究了FGF7在乳腺中的作用。结果表明:FGF7主要定位于乳腺导管上皮细胞以及腺泡上皮细胞外周,同时在基质中有弱的表达。KGFR主要出现在导管上皮细胞以及腺泡上皮细胞上,在乳腺发育的个别时期细胞外基质中有弱表达。FGF7在退化4d达到最高值;KGFR在泌乳4d达到最高值。除青春期和妊娠期外,添加FGF7均能显著诱导乳腺上皮细胞产生KGFR。FGF7能促进小鼠乳腺上皮细胞的增殖分化,降低乳腺上皮细胞的细胞凋亡。
To invest the dynamic changes in expression and localization of fibroblast growth factor 7（FGF7）and its receptor in mammals devel- opment, lactation and involution; reveal the relationships between the expression of FGF7 and its receptor and the function, immunofluorescence assay were used to detect FGF7 by a laser scanning confocal microscope firstly in mammary gland slices of Kunming mice, explants of mammary gland were cultured to invest the function of FGF7 in mammary gland. The results revealed that： FGF7 mainly localized in the peripheral of mammary gland ductal epithelial cells and acinar cells , and a weak expression in matrix. KGFR occurred mainly in the ductal epithelial cells and acinar cells, and a weak expression in matrix in the individual time.The expression of FGF7 was highest in involution 4d and the expression of KGFR was highest in lactation 4d. Except virgin and pregnancy, FGF7 treatment could significantly induced mammary ep- ithelial to produce KGFR.FGF7 promoted epithelial proliferation and differentiation of mouse mammary and reduce the apoptosis in mammary epithelial cell.
出处
《中国乳品工业》
CAS
北大核心
2008年第1期26-31,共6页
China Dairy Industry
