碱解增敏荧光法测定牛奶中四环素残留

Fluorescent spectrophotometric determination of hydrolyticresidue tetracycline sensitized by cetyltrimethylammonium bromide

四环素在浓度为0.1mol/L的NaOH溶液中反应,生成强荧光降解产物异四环素,在碱性条件下加入十六烷基三甲基溴化铵后,荧光强度增大两倍左右。基于此,建立了测定牛奶中四环素残留的新方法。线性动态范围为50～2500μg/L;检出限2.06μg/L;样品的加标回收率为84.56%～90.06%。本法成功的用于牛奶中四环素的残留测定。

Tetracycline can be hydrolyzed as strong fluorescent iso-tetracycline in 0.1mol/L NaOH solution. The fluorescent intensity of the hydrolyzate sensitized by cetyltrimethyl- ammonium bromide （CTAB） is about 2-fold of iso-tetracycline itself. A new determination method for residue tetracycline in milk is developed by the principle. The linear dynamic range is fi＇om 50μg/L to 2 500 μg/L, detection limit is 2.06 μg/L, and the recoveries of spiked sample are between 84.56% and 90.06%. The method was successfully applied to the determination of residue tetracycline in milk.