人参环氧炔醇对体外培养施万细胞表达神经营养因子的影响及可能机制的研究

EFFECT AND POSSIBLE MECHANISMS OF PANAXYDOL ON NTFs EXPRESSION IN SCHWANN CELLS IN VITRO

目的研究人参环氧炔醇（PND）对体外培养施万细胞（SCs）神经营养因子（NTFs）表达的影响;并探讨其机制。方法用含有不同浓度的PND培养液分别处理SCs,以免疫组织化学法检测PND对SCs NTFs表达的影响;放射免疫方法测定SCs内环磷酸腺苷（cAMP）水平的变化,并进行图像处理及统计学分析。结果PND分别在2.5-20.0μmol/L及5.0--20.0μmol/L剂量范围内可分别促进体外培养SCs NGF及大脑衍生神经营养因子（BDNF）的表达（P〈0.05）,在10μmol/L剂量对两者促进作用均最明显（P〈0.01）;在2.5--20.0μmol/L剂量范围内PND可提高体外培养SCs胞内cAMP含量（P〈0.05）,而在10μmol/L剂量作用最显著（P〈0.01）。结论PND能明显促进体外培养SCs NGF、BDNF的表达和分泌;PND可提高SCs的胞内cAMP水平,并与PND促进体外培养SCs NGF、BDNF表达之间存在相关性,提示其可能是PND提高体外培养SCs NGF、BDNF表达的调控机制及信号传导通路。

Objective To study the effects of panaxydol（PND） upon neurotrophic factors （NTFs） expression in Schwann cell, and to further reveal the mechanisms that underlie these effects. Methods Schwann cells were treated with PND of different concentrations in the culture medium. NTFs expression was examined through immunoiistochemistry assay. The intracellular cAMP level was assessed by radioimmunologic assay. Results There was an increase in NGF expression after PND treatment within the range of 2.5-20.0μmol/L （P〈0.05） and an increase in brain derived neurotrophic factor（BDNF） expression after PND treatment within the range of 5.0-20.0μmol/L （P〈0.05）. Both NGF and BDNF expressions peaked in 10μmol/L PND group with P〈0.01, which was considered to be statistically significant; Intracellular cAMP increased as well after PND treatment within the range of 2.5-20.0μmol/L （P〈0.05） and the intracellular cAMP level also peaked in the 10μmol/L PND group （P 〈 0.01）. Conclusion PND treatment of Schwann cell enhances NGF and BDNF expressions in vitro. In addition, an increase in the intracellular cAMP level was observed after PND treatment and that correlates with NGF and BDNF expression enhanced by PND, which indicates the possible mechanisms involved in PND functions on NTFs expression.