摘要
目的通过检测胃癌细胞系SGC7901/VCR、SGC7901和BGC-823,以及永生化胃上皮细胞系GES中B7-H1的表达,探讨B7-H1与胃癌的发生及多药耐药(MDR)的关系。方法上述细胞系培养于含10%灭活胎牛血清的RPMI-1640培养基中,利用RT-PCR技术、细胞免疫化学染色以及细胞免疫荧光染色技术与流式细胞术检测4种细胞系中B7-H1 mRNA与B7-H1蛋白的表达情况,并比较其在4种细胞系中的表达强度。结果B7-H1 mRNA在4种细胞系中均有表达,表达强度按照GES-1、BGC-823、SGC-7901、SGC-7901/VCR的顺序递增;细胞免疫化学染色与免疫荧光染色表明,B7-H1蛋白主要表达在上述细胞的细胞膜和少量细胞浆中,结合流式细胞术检测进一步证实,B7-H1蛋白在4种细胞系中的表达结果与mRNA的表达一致。结论B7-H1表达在胃上皮细胞上,可能通过抑制机体免疫反应,促进胃癌细胞生长及MDR基因表达。
Objective B7-H1 is a recently identified co-stimulatory molecule expressed on APCs and can inhibit the activation and function of T lymphocytes. This research is to study if B7-H1 has a relationship with carcinogenesis and MDR of gastric carcinoma by examining the expressions of B7-H1 in human gastric carcinoma cell hnes SGC7901/VCR, SGC7901, BGC-823 and in the permanent gastric epithelial cell line GES-1. Methods Cells were cultured in RPMI-1640 supplemented with 10% fetal calf serum. The B7-HI mRNA was detected by RT-PCR and the protein of B7-H1 was detected by cytoimmunoehemistry staining, cytoimmunofluoreseence staining and flow cytometric analysis. The expression levels of B7-H1 mRNA and the protein of B7-H1 in the above mentioned four kinds of cell lines were compared. Results All the four cell lines express B7-H1 mRNA at different levels. And B7-H1 expression level increased in the order of from GES-1 to SGC7901/VCR cell line, BGC-823 and then to SGC7901 cell line. The protein of B7-H1 was found to be distributed in the cell membrane and in a little cytoplasm in all the four cell lines through techniques of cytoimmunochemistry staining and cytoimmunochemistry staining. Further more with the result of flow cytometric analysis, the protein expression level of B7-H1 in all these cell lines was found to be consistent with B7-H1 transcript level expression. Conclusion The results suggest that B7-H1 expression on gastric epithelial cells may promote the growth of gastric carcinoma cells and the expression of MDR gene through inhibiting host immune responses.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第1期70-73,共4页
Acta Anatomica Sinica
基金
教育部新世纪优秀人才支持计划(NECT-05-0609)
河南省重大科技攻关项目资助(0222031300)
