一种基于大鼠颈髓连续切片的计算机三维重建方法

AN COMPUTERIZED 3D RECONSTRUCTION METHOD BASED ON RAT'S CERVICAL CORD SERIAL SECTIONS

目的以个人计算机(PC)为平台,探讨一种基于大鼠颈髓切片进行脊髓三维重建方法。方法制作正常SD大鼠颈髓节段包埋蜡块,于颈髓标本四周进行外定位标记,Leica石蜡切片机进行连续横断切片,每切3张,用CANON数码相机对蜡块中的颈髓标本及其外定位标记进行摄片,获得连续切片图像。所得图像进行排序,裁切,转换为灰度图像并进行背景不均匀校正后,利用3D-DOCTOR软件对每张图像上的颈髓、颈髓灰质及定位孔进行边缘提取,利用定位孔对连续图像进行自动配准,三维表面重建后在PC机上进行任意角度观察、切割和测量。结果利用大鼠颈髓连续切面图像重建得到大鼠颈髓白质和灰质,并对其进行测量,获得了表面积和体积等数据。结论利用石蜡包埋和"硬"定位技术可以获得颈髓节段连续横断切面图像,进行三维重建并对重建结构进行自由观察和测量。

Objective With personal computer （PC） as the platform, to explore an computerized 3D reconstruction method based on rat＇s cervical cord serial sections. Methods Normal cervical cord segment of SD rat was embeded in paraffin block, four locating marks were made at four comers around the specimen, the specimen was sectioned serially with Leica paraffin microtome, after each three sections, the images of specimen and outer locating marks were taken with CANON digital camera and the serial sections were obtained. The obtained images were ordered, cut, turned into gray scale images and background uneven corrected. The edges of white matter, grey matter and locating marks in each image were extracted, the locating marks were used for automated registration of serial sections with 3D-DOCTOR software, the 3D surface reconstruction of cervical cord segment was observed, cut and measured freely in PC. Results The grey matter, white matter of rat cervical cord segment were reconstructed from serial transverse sections, the reconstructed segment was measured to obtain the surface area and volume data. Conclusion The paraffin embedding and ＂hard＂ locating marks techniques can be used to obtain the images of serial transverse sections of rat＇ s cerival cord segment, to make 3D reconstruction which can be observed and measured freely.