白藜芦醇对大鼠慢性阻塞性肺疾病的干预作用及其机制

Effect of resveratrol on chronic obstructive pulmonary disease in rats and its mechanism

研究白藜芦醇对大鼠慢性阻塞性肺疾病的作用及可能的机制。单次气管注射博莱霉素(BLM)及连续27d烟熏建立大鼠COPD模型,检测动脉血气参数变化、肺功能指标,测定支气管肺泡灌洗液(BALF)中细胞间粘附分子-1(ICAM-1)含量,检测肺湿/干重(W/D)比值,观察组织病理学变化。实验发现,白藜芦醇能抑制大鼠COPD的PaO2的下降,升高FEV0.3/FVC的比值,降低BALF中ICAM-1的含量,降低肺W/D比值,减轻肺组织病理学损伤。结果提示,白藜芦醇对大鼠COPD具有干预作用,其作用机制可能与抑制炎症因子的释放有关。

The purpose of this study is to establish COPD animal model by intra-tracheal instillation of bleomycin （BLM） once and exposure to cigarette smoke for continuous 27d, and to observe the effects of the inhalation on the model. At the 29th day, blood samples were taken from cervical artery for blood-gas analysis and parameters of lung function were recorded. Bronchoalveolar lavage fluid （BALF） was collected to measure intercellular adhesion molecule-1 （ICAM-1） concentration. The results showed that atomization inhaled resveratrol could alleviate rat COPD lung injury accompanied by amelioration of pathological changes, increase the ratio of forced expiratory volume in 0.3 s （FEV0.3 ） and forced vital capacity （FVC）, and decrease the ICAM-1 level in BALF. The ultimate reduction of inflammatory factors was involved, at least in part, in the mechanism of resveratrol effects.