UPLC-UV测定细辛地上和地下部位中的马兜铃酸A

Analysis of aristolochic acid A from the aerial and underground parts of Asarum by UPLC-UV

马兜铃酸因被证实具有肾毒性而引起广泛的重视，尤其近年来美国FDA有关法令中，已明确提出禁止使用含有马兜铃酸的药物，因此马兜铃科植物的安全性受到质疑。中药细辛是临床上常用的祛风止痛药，属于马兜铃科，在2005版之前的历版中国药典，

To develop an UPLC-UV method for the determination of aristolochic acid A in the aerial and underground parts of Asarum, an AcquityUPLC^TM HSST3 column （50 mm×2.1 mm ID, 1.8 μm） was used with the mobile phase of acetonitrile-0.1% FA and eluted in gradient mode. The flow rate was 0.5 mL·min^-1 , the detection wavelength was 251 nm, and the column temperature was 40 ℃. The calibration curve was linear in the range of 18-1450 ng of aristolochic acid A with correlation coefficient of 0.999 9 and the minimum detective concentration was 18.25 ng·mL^-1 . The method developed is accurate and reproducible, and can be applied in the determination of aristolochic acid A in Asarum.