人参皂甙Rg1对去卵巢帕金森病模型大鼠黑质多巴胺能神经元的保护作用被引量：11

THE PROTECTIVE EFFECT OF GINSENOSIDE RG1 ON DOPAMINERGIC NEURONS OF SUBSTANTIA IN THE OVARIECTOMIZED RAT MODEL OF PARKINSON'S DISEASE

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摘要目的:探讨人参皂甙Rg1对6-羟基多巴(6-OHDA)制备的去卵巢(OVX)帕金森病(PD)模型大鼠黑质(SN)多巴胺能神经元的保护作用及其可能机制。方法:应用6-OHDA制备的OVX PD模型大鼠,侧脑室给予Rg1或雌激素。免疫组织化学染色酪氨酸羟化酶(TH)阳性神经元和Bcl-2蛋白。Perls’铁染色检测SN铁含量。结果:①Rg1或雌激素可抑制阿朴吗啡诱导的PD大鼠旋转行为;②在损毁侧SN,Rg1或雌激素用药组TH阳性神经元数量较6-OHDA组显著增多;③6-OHDA组损毁侧SN内铁含量较健侧明显升高,应用Rg1或雌激素后,SN铁含量较模型组明显减少;④与6-OHDA模型组相比,Rg1及雌激素均可增加损毁侧大鼠SN内Bcl-2蛋白表达。结论:人参皂甙Rg1具有类雌激素样作用,对OVX PD模型大鼠黑质DA能神经元有明显的保护作用,其作用机制可能与降低铁负载和抗凋亡有关。 Aim： To investigate the neuroprotective effect of ginsenoside Rgl on dopaminergic neurons of substantia nigra in ovariectomized rat model of Parkinson＇ s disease and the possible mechanisms. Methods： Wistar female rats were ovariectomized and treated with vehicle, ginsenoside Rgl or 17-13 estradiol intracerebroventricularly in the 6-OHDA induced rat model of Parkinson＇s disease. Immuno-histochcmistry was used to detect the tyrosine hydroxylase （TH） immunoreactive neurons and the protein expression of Bcl-2. Perls＇ iron staining was used to determine the changes of iron in substantia nigra （SN）. Results：（1）Rgl or 17-0 estradiol treatment could ameliorate the rat＇s rotational behavior induced by apomorphine. （2）Rgl or 17-β estradiol treatment could increase TH immunoreactive neurons in the injured side of SN compared to the 6-OHDA group. （3）Iron staining in the injured side of SN was significantly increased comparing with the contralateral side in the 6-OHDA group. Rgl or 17-β estracliol treatment could reverse the increase of iron staining. （4）Both Rgl and 17-β estradiol treatment could increase Bcl-2 protein expression in the injured side of SN compared to the 6- OHDA group. Conclusion： Ginsenoside Rgl has estrogen-like activities and has neuroprotective effects on the dopaminergic neurons in the 6-OHDA induced ovariectomyzed（OVX） rat model of parkinson＇ sldisease（PD）. This effect may be attributed to attenuating iron overload and anti-apoptosis.

作者徐丽刘黎星陈文芳谢俊霞黄文秀

机构地区青岛大学医学院生理学教研室香港理工大学

出处《中国应用生理学杂志》 CAS CSCD 北大核心 2008年第1期1-5,共5页 Chinese Journal of Applied Physiology

基金国家自然科学基金资助项目(30570573) 山东省教育厅资助项目(J05L01) 青岛市科学技术局资助项目(05-10-JC-9705-2-JC-136)

关键词人参皂甙RG1 雌激素帕金森病黑质 6-羟基多巴铁 Bcl-2 ginsenoside Rgl estrogen parkinson＇s disease substantia nigra 6-OHDA iron Bcl-2

分类号 Q426 [生物学—神经生物学] R592 [医药卫生—老年医学]

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