Cu(2＋)对离体培养猪腺垂体细胞GH分泌的影响

EFFECTS OF Cu^(2+) ON GROWTH HORMONE SECRETION OF PIG PITUITARY CELLS IN CULTURE

目的:研究铜离子(Cu2+)对离体培养猪腺垂体细胞生长激素(GH)分泌的影响。方法:试验选取32-35日龄仔猪腺垂体细胞,于10%小牛血清的Dulbecco MEM培养液(DMEM)中离体培养48 h。之后用含不同浓度Cu2+(0 mg/L、0.025 mg/L、0.1 mg/L、0.4 mg/L、1.6 mg/L)的无小牛血清DMEM培养液培养36 h,于12 h、24 h、36 h收集细胞培养液,用Linco公司的试剂盒及放射免疫法测定培养液中GH浓度。结果:铜离子离体培养腺垂体细胞24 h,0.025 mg/L、0.1 mg/L、0.4 mg/L组培养液中GH浓度均高于0 mg/L组,其中0.1 mg/L组与0 mg/L组差异显著(P<0.05)。结论:铜离子可促进离体培养猪腺垂体细胞分泌GH。

Aim： To study the effects of Co2＋ on growth hormone （GH） secretion of pig pituitary cells in vivo. Methods： Gland pituitary cells of 32-35 days old pigs were incubated for 48 h in presence of 10% fetal calf serum in DMEM. Cells were treated for 36 h with various concentrations of Cu2＋ （0 mg/L, 0.025 mg/L, 0.1 mg/L, 0.4 mg/L, 1.6 mg/L） in serum-free DMEM. Culture medium was collected in 12 h, 24 h, 36 h thereafter for GH measurement and the radioimmunoassay kits were provided by Linco. Results： The GH concentration was higher in treatments of 0.025 mg/L,0.1 mg/L,0.4 mg/L than that of 0 mg/L when the cells were treated with CU2 ＋ for 24 h, and it was significandy different （P 〈 0.05） between treatment 0.1 mg/L and treatment 0 rng/L. Conelusion： It was concluded that the presence of Cu2＋ stimulated pig pituitary cells to secret GH in vivo.