催乳素对佐剂关节炎大鼠滑膜组织MMP-9表达的影响

THE EFFECT OF PROLACTIN ON THE EXPRESSION OF MATRIX METALLOPROTEINASE-9 IN THE SYNOVIUM OF ADJUVANT ARTHRITIS RATS

目的:探讨催乳素(PRL)对佐剂关节炎(AA)大鼠滑膜组织基质金属蛋白酶-9(MMP-9)表达的影响,为进一步阐明PRL在类风湿性关节炎(RA)发病过程中的作用机制及为RA的治疗提供实验依据。方法:雄性Wistar大鼠32只分为4组(n=10):①正常对照组(A组)(n=10);②佐剂关节炎对照组(B组)(n=10);③高催乳素血症佐剂关节炎组(C组)(n=10);④低催乳素血症佐剂关节炎组(D组)。放射免疫法测定各组血清PRL含量;明胶酶谱法测定MMP-9的活性;免疫组织化学方法检测各组滑膜组织MMP-9免疫反应变化;Western blot测定各组滑膜组织MMP-9的表达。结果:B组滑膜组织MMP-9的活性及表达明显高于A组,C组MMP-9的活性及表达最高,D组较B组有所减少,但仍高于A组。结论:PRL影响滑膜组织MMP-9分泌是其影响RA发病过程的一种作用机制。

Aim： To determine the exact roles of prolactin（PRL） in the pathogenesis of rheumatoid arthritis（RA） and supply experimental basis for clinical treatment of RA, and to investigate the expression of matrix metalloproteinase-9（MMP-9） in the synovium of adju- vant arthritis rats.Methods： Forty rats were divided into four groups（ n = 10）： （1）Normal control group（group A）; （2）Adjuvant arthritis control group（group B）; （3）Hyperprolactinemic adjuvant arthritis group（group C）; （4）Hypoprolactinemic adjuvant arthritis group（group D）. The content of PRL in the serum was detected by radio-immunoassay method. The activity of MMP-9 was analyzed by gelatin zymography. The alteration of MMP-9 immunoreactivity were investigated by means of immunohistochemistry in the synovium of all groups. The expressions of MMP-9 were investigated by Western blot in the synovium of all groups.Results： Compared with group A, the activity and expression of MMP-9 of group B in the synovium were highly increased. The activity and expression of MMP-9 in the synovium were the most distinctive in group C. Compared with group B, the activity and expression of MMP-9 in the synovium were decreased in group D, but still higher than group A.Conclusion： The present results indicated that PRL might involved in the pathogenesis of RA by regulating the secretion of MMP-9 in the synovium.