共振光散射法研究甲萘威与DNA的作用及其应用

Study on the Interaction of Carbaryl and ctDNA by Resonance Light Scattering Spectra and Its Application

通过共振光散射光谱(RLS)和电子吸收光谱的特征,探求了甲萘威与ctDNA的结合方式,实验表明在pH1.97的条件下,甲萘威与ctDNA既有表面聚集又有嵌入式结合的双重作用,结合形式与二者之间的浓度比有关。在此条件下,甲萘威与ctDNA作用的RLS强度与ctDNA的浓度呈线性关系,据此建立了一种简单快速测定ctDNA的新方法。ctDNA的浓度在0.02～3μg.mL-1的范围内与RLS强度呈良好的线性关系,线性方程为I=200.77c(μg.mL-1)+118.91,相关系数r=0.9989。该方法已成功地用于人工混合样品的测定。

Interaction modes of carbaryl and DNA were studied by resonance light scattering （RLS） spectra and absorption spectra. Experiments show that at pH 1.97, there are two interaction modes between carbaryl and ctDNA, namely surface assembly mode and intercalative mode. Interaction mode has a relation with the concentration ratio of carbaryl to ctDNA. Under this condition, the RLS intensity of carbaryl and ctDNA is proportional to the concentration of ctDNA. According to this, one new simple and rapid method of determining ctDNA was set up. The linear ranges of the concentration of ctDNA were 0.02-3 μg· mL^-l , the linear regression equation was I= 200. 77c（μg·mL^-1 ） ＋ 118. 91, and the correlation coefficient was r= 0. 998 9. This method has successfully been used to determine the synthetic samples.