兔骨髓间充质干细胞向雪旺细胞体外分化的实验研究

Differentiation of rabbit bone mesenchymal stem cells into Schwann cells： an in vitro study

目的研究兔骨髓间充质干细胞（mesenchymal stem cells，MSCS）体外分化为雪旺细胞（sehwann cells，SCS）的可行性，并对其功能进行检测。方法取第3代生长状态良好的MSC，诱导组经β-巯基乙醇和维甲酸序贯诱导4d后用含富血小板血浆（platelet—rich plasma，PRP）的培养基培养7d，荧光染色对SC标志物S-100进行鉴定，并于第4、7、9和11天在换液前收集培养液，ELISA法检测神经生长因子（never growth factor，NGF）的含量，并和空白对照组比较。结果体外诱导的MSC发生形态改变并表达S-100蛋白，诱导组在第4、7、9、11天时NGF的含量分别为[（254±25）pg／ml，x±s，下同]、（372±31）pg／ml、（485±29）pg／ml、（534±32）pg／ml，均明显高于对照组，差异有统计学意义（P〈0．01）。结论MSC体外能诱导分化为SC，且具有分泌NGF的功能。

Objective To explore the possibilities of mesenchymal stem cells （MSCs） differentiating into Schwann cells （SCs） and examine its bioactivity. Methods Third generation MSCs were induced by β-ME and RA censecutively for 4 days and then cultured with platelet rich plasma （PRP） for 7 days. SC marker S-100 was used to discriminate the MSC properties by fluorescent staining. The centents of NGF in the culture fluid was measured at 4th, 7th, 9th and llth day by ELISA. Results MSCs changed morphologically into cells resembling SCs after in vitro induction and expressed S-100. The NGF centents（pg/ml） of the induction group were 254 ± 25,372±31,485±29 and 534±32 at 4th, 7th, 9th and llth day, respectively. This NGF content obviously exceeded the centent of the control group（ P 〈 0.01 ）. Conclusion These results suggested that MSCs can differentiate into SCs in vitro and excrete NGF.