摘要
目的探讨环加氧酶2（cyclooxygenase-2，COX-2）在人神经胶质瘤细胞株SHG44发生多药耐药过程中的作用和机制。方法应用阿霉素浓度递增和间歇诱导法建立SHG44／ADM耐药株，采用过表达COX-2和COX-2特异抑制剂NS398分别干预SHG44／WT和SHG44／ADM细胞株，免疫印迹法检测COX-2和MDR-1的表达量，酶联免疫分析PGE2产生量，荧光分光光度计检测细胞内阿霉素浓度。结果过表达COX-2增加细胞PGE2生成量，同时增加MDR-1表达和活性；NS398抑制细胞PGE2生成量，减少MDR-1表达和活性。结论COX-2参与神经胶质瘤细胞的多药耐药。
Objective To understand the molecular mechanism and effect of cyclooxygenase-2 on muhidrug resistance of human glioma SHG44 cell line. Methods Stepwise concentration increasing and intermittent administration method was used to construct SHGd4/ADM, over expressed COX-2 and the COX- 2 specific inhibitor NS398 were used to treat SHG44/WT and SHG44/ADM, the expression of COX-2 and MDR-1 were detected by western blotting, the production of PGE2 was assayed by enzyme immunoassay, ADM accumulation was determined using fluorescence spectrometry. Results COX-2 overexpression increased PGE2 production and MDR-1 protein expression and activity. NS398 was able to block the production of PGE2 and decrease MDR-1 expression and activity. Conclusion COX-2 mediates mutildrug resistance by MDR-1 expression and activity.
出处
《中华神经外科杂志》
CSCD
北大核心
2008年第2期149-152,共4页
Chinese Journal of Neurosurgery
基金
国家自然科学基金资助（30500463）
