酸樱桃组培快繁技术体系的研究

In vitro Tissue Culture and Rapid Propagation of Sour Cherry

以酸樱桃(Prunus cerasus)新梢茎尖和带腋芽的茎段为外植体,采用茎段组织培养的方法,用MS和F14培养基,分别添加不同质量浓度的6-苄基氨基腺嘌呤(6-BA)和3-吲哚丁酸(IBA),进行酸樱桃组培快繁技术体系研究。结果表明,MS培养基是适合酸樱桃生长的基本培养基;培养基中分别加入0.5 mg.L-16-BA和0.2 mg.L-1IBA,增殖效果最好,增殖率为4.46倍,有效新梢数可达83.6%;组培苗在1/2MS+0.5 mg.L-1IBA培养基上根诱导效果最好,生根率可达71.4%,平均根长2.1 cm;移栽到蛭石基质中成活率可达80%。

The technology of tissue culture and rapidly propagation of sour cherry was studied using new shootings and stem sections with axillary bud as explants. There were 2 kinds of basal cultural medium （MS and F14）,2 kinds of hormone with different concentration [6-benzyladenine （6-BA） and 3-indole butyric acid （IBA）]. The results showed that the buds grown better in MS than that in F14 medium. MS medium with 0.5 mg · L^-1 6-BA and 0.2 mg · L^-1 IBA was the most suitable for the propagation of sour cherry and the propagation rate reached 4.46 times. The effective number of new shoot was up to 83.6%. On the other hand,the good medium to induce root was 1/2 MS＋0.5 mg · L^-1 IBA with 71.4% of rooting rate and 2.1 cm of average root length. There was higher proportion of viability planted test-tube seedlings in the substratum （vermiculite）,the survival rate was up to 80%.