叶绿体蛋白质组研究进展

Research Progress in Chloroplast Proteome

亚细胞蛋白质组学是近年来蛋白组学研究中的一个热点。通过细胞器的纯化和亚细胞组分的分离,降低了样品的复杂性,增大了相应蛋白质组分的富集,有利于由此分离获得的蛋白质的序列分析及功能鉴定。叶绿体蛋白质组为植物亚细胞蛋白质组学研究中相对全面的一部分,利用亚细胞分离结合双向电泳技术系统地鉴定叶绿体中蛋白质组分是获取叶绿体蛋白质信息、确定其功能的重要技术手段。本文就近年来植物叶绿体蛋白质组涵盖的叶绿体内、外被膜、叶绿体基质、类囊体膜和类囊体腔蛋白的研究进行综述,以全面认识叶绿体蛋白的组成、特点及其在叶绿体生理生化代谢网络中的作用。

Subcellular proteomics is currently the most effective approach to characterize subcellular compartments. Based on the powerful combination of subcellular fractionation with proteomic analysis, it makes possible to characterize all chloroplast proteins completely. Chloroplast proteome is one of the most intensive aspects of the plant subcellular proteomics research. By using subcellular proteome technique,more and more choloplastic proteins have been identified. The present review summarizes the recent progress in proteins located in the different chloroplastic compartments,including the inner and outer envelop membranes, chloroplast stroma,thylakoid membranes and thylakoid lumen. We hope it contributes to understand the characters of chloroplastic proteins and their functions in the chloroplastic metabolism networks.