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Hedgehog信号通路特异性抑制剂cyclopamine对HaCaT细胞白介素8分泌的影响 被引量：1

Influence of cyclopamine, a specific inhibitor of Hedgehog pathway, on the secretion of interleukin 8 in HaCaT cells
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摘要 目的:探讨Hedgehog信号通路特异性抑制剂cyclopamine对人正常角质形成细胞HaCaT细胞白介素(IL)-8分泌的影响。方法:在HaCaT细胞培养基中加入cyclopamine,采用反转录(RT)-PCR和ELISA方法检测HaCaT细胞在cyclopamine处理后不同时段IL-8 mRNA及其分泌的变化。结果:HaCaT细胞在cyclopamine处理后IL-8的mRNA表达及其分泌均减少(P<0.05)。结论:Hedgehog信号通路特异性抑制剂cyclopamine可抑制HaCaT细胞分泌IL-8。 Objective： To investigate the influence of cyclopamine, a specific inhibitor of Hedgehog pathway, on the secretion of interleukin 8 （IL-8） in HaCaT cells. Methods： RT-PCR and ELISA were employed to measure the secretion of IL-8 in HaCaT cells at different time after treatment with cyclopamine. Results： IL-8 mRNA expression was significantly inhibited in HaCaT cells treated with cyclopamine, and IL-8 secretion was also reduced （P 〈 0.05）. Conclusion： Cyclopamine, a specific inhibitor of Hedgehog pathway, can suppress the IL-8 secretion of HaCaT keratinocytes.
作者 周扬 刘海燕 刘玉峰 李承新
机构地区 第四军医大学全军皮肤病研究所
出处 《临床皮肤科杂志》 CAS CSCD 北大核心 2008年第3期157-159,共3页 Journal of Clinical Dermatology
基金 国家自然科学基金资助项目(30572099) 教育部新世纪优秀人才资助计划资助项目
关键词 HEDGEHOG信号通路 CYCLOPAMINE 角质形成细胞 ELISA RT-PCR Hedgehog cyclopamine HaCaT cells ELISA RT-PCR
分类号 R285.5 [医药卫生—中药学]
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参考文献10

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共引文献63

同被引文献15

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临床皮肤科杂志
2008年 第3期

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