酶联免疫吸附试验夹心法检测解脲脲原体方法的建立

An antibody-sandwich enzyme linked immunosorbent assay to detect Ureaplasma urealyticum

目的为了提高解脲脲原体(Ureaplasma urealyticum,UU)检测的快速性。方法用酶联免疫吸附试验(ELISA)夹心法检测UU抗原并与传统的培养法相比较。结果ELISA夹心法敏感度为92.6%,特异度为97.4%,最低能够检测出蛋白含量为5～10ng/ml的UU抗原。结论ELISA夹心法是一种敏感、方便、快捷、适合大规模标本检测解脲脲原体的方法。

Objective To establish an antibody-sandwich enzyme linked immunosorbent assay （ELISA） for the detection of Ureaplasmaurealyticum （UU）.Methods We used an antibody-sandwich ELISA to detect UU and compared the results with cell culture method. Results The rate of sensitivity of the ELISA was 92.6% and the rate of specificity was 97.4%. The lowest detection limitation was 5 10 ng/ml of UU antigen. Conclusion The antibody-sandwich ELISA is a sensitive, convenient and fast method for detection of UU for the screening of large numbers of specimens.