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用于分子生态学研究的鹅盲肠微生物总DNA提取方法

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摘要 采用8种方法提取了鹅盲肠微生物总DNA,通过PCR扩增细菌16S rDNA片段的V3区,并对8种DNA提取方法的可行性进行后续分子操作检验。结果表明,方法3提取的鹅盲肠微生物总DNA较好,8种方法通过PCR扩增均能获得较亮的扩增产物,并能通过PCR-SSCP对盲肠微生物多样性进行分析。
出处 《江苏农业科学》 CSCD 北大核心 2008年第1期42-44,共3页 Jiangsu Agricultural Sciences
基金 江苏省"十五"科技攻关项目(编号:BE2001369)
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