木瓜复合抗氧化物颗粒的抗氧化特性研究

Research of Antioxidant Properties of Compound Antioxidant Constituent Particles Derived from the Chaenomeles

采用纤维素酶解法、γ-环糊精包合以及新型喷雾制粒工艺将木瓜中所含主要的抗氧化成分从植物组织中转移到具有良好胃肠道给药性的环糊精载体中,制作成木瓜复合抗氧化物颗粒,其中维生素C含量下降了22.7%,为2.9 mg/g;多酚含量下降了14.7%,为8.1 mg/g;通过2,2-二苯基苦基苯肼自由基(DPPH.)微孔板定量法测定了木瓜酶解上清液CES、木瓜沉淀醇提液CEr和木瓜复合抗氧化物颗粒CACP的总抗氧化能力(Total Antioxi-dative Capability)及化学动力学曲线.根据动力学稳定时相点及统计学分析得:CES的TAC为82.05μg DPPH./mL(p<0.05),CEr的TAC为38.62μg DPPH./mL(p<0.05),CACP为609.8μg DPPH./g(p<0.05).

Compound Antioxidant Constituents Powder （CACP） is made by transferring the antioxidant constituents from the Chaenomele fruit into the Schardinger dextrin through the processes of cellulase enzymolysis, g-cyclodextrin clathration and spraying granulation. Sufficiently high content of vitamin C （2. 9 mg/g powder） and phenols （8. 1 mg/g powder） remained in CACP. Microplate quantification assay was used to examine the scavengers＇ kinetic curve and determine their total antioxidative capability （TAC）, which represented amount （μg） of DPPH· scavenged by every gram or milliliter of the centrifugal supernatant （TAC=82. 05 mg DPPH·/mL）, ethanol extract from the residue （TAC=38. 62 mg DPPH·/mL） and CACP （TAC=609.8 mg DPPH·/g in stable） with highest value of scavenging DPPH·（（p〈0.05））.