晋旱125×(昔野×501)杂交后代保护酶与抗旱性关系的研究

Research on Relationship between Protecting Enzyme and Drought Resistance of Hybrid Descendents of Jinhan125×(Xiye×501)

选用晋旱125×(昔野×501)杂交组合的亲本及后代所产生的有代表性的四个特殊类型73-1(尖叶白花)、73-2(圆叶紫花)、73-3(圆叶白花)、73-4(尖叶紫花)为试验材料,分别在正常供水和干旱胁迫的条件下,测定苗期和花荚期的POD、SOD、CAT等与抗旱性相关的保护酶指标,并对株高、株荚数、百粒重等农艺性状进行综合分析,还对亲本和子代的抗旱性差异情况用抗旱性隶属函数值(D值)进行了综合的测评,结果表明:干旱胁迫下,不同品系的保护酶活性均有所变化,且在不同时期,各种酶的变化程度不同;在大豆对水分最为敏感的花荚期对SOD、POD、CAT的活性进行测定和分析,采用抗旱隶属函数值的方法综合测评,认为这一群体中抗旱性从强到弱的次序为:晋旱125、73-2(圆叶紫花)、昔野×501、73-1(尖叶白花)、73-3(圆叶白花)、73-4(尖叶紫花)。

In this paper,four special representative types from a cross between Jinhan125（Xieye 501） were selected as test materials, they were 73-1 （sharp leaf and white flower）, 73-2 （round leaf and purple flower）, 73-3 （round leaf and white flower）,73-4（sharp leaf and purple flower）. In the study,in the condition of normal water supply and drought stress,indexes of protecting enzyme relevant to drought-resisance in the periods of young plant and flower-podding were determined. The indexes were activities of POD, SOD, CAT. Additionally, agricultural characters such as height of plant,total pods, hundred-seed weight and so on were comprehensively analyzed. Also, drought-resistant difference between parents and descendants was comprehensively evaluated by subordinate function value, the results showed： In the condition of drought stress, activity of protecting enzymes of various varieties changed and the changing extents of enzymes were distinct in different periods. So, activity of protecting enzyme could be utilized as one of the indexes compressively evaluating drought-resistance of soybeans. During the breeding period,the flower-podding period was the most sensitive to water content, in this period, activities of POD, SOD and CAT were determined and analyzed. Simultaneously,the method of drought-resistant subordinate function value was employed to assess comprehensively. The order of drought-resistance of the group was considered as following： Jinhan125,73-2（round leaf and purple flower）,（Xiye 501）,73-1（sharp leaf and white flower）,73-3 （round leaf and white flower）,73-4（sharp leaf and purple flower）.