湖北省肾综合征出血热血清学分型

Serotyping of Hemorrhagic Fever with Renal Syndrome in Hubei Province

为找到湖北省肾综合征出血热（ＨＦＲＳ）疫源地分型的血清学证据，在对全省ＨＦＲＡ疫源地进行流行病学初步分型的基础上，按流行病学要求，在全省不同动物地理区划、不同发病强度的疫区内，用微量细胞病变中和试验对９２份免疫荧光试验初筛阳性的病人血清进行血清学分型。发现其中ＨＴＮ型（Ⅰ型）４８例，占５２．１８％，ＳＥＯ型（Ⅱ型）２９例，占３１．５２％，未定型１５例，占１６．３０％；全省疫区按血清学划分属于Ⅰ型为主的混合型疫区，但不同小区血清学类型各有特点；老疫区的型别分布更复杂，因此本省ＨＦＲＳ疫苗的运用最好用双价疫苗，或选用与当地型别一致的单价疫苗；本次血清分型结果与流行病学分型结果基本相符，由于疫区型别在不断演变，所以应定期进行分型调查；在进行疫苗效果观察时，还应注意先后接种两型疫苗的接种反应。

Ninety two serum specimens, positive for antibodies against hemorrhagic fever with renal syndrome (HFRS) virus in initial screening with immunofluorescence assay technic (IFAT), were serotyped with micro cell pathogenic effects neutralization test based on preliminary epidemiological classification of epidemic foci of HFRS throughout the province to find out serological evidence of HFRS typing in Hubei Province. It was found that 48 of the specimens were belonged to Type I (HTN) accounting for 52.18 percent, 29 Type II (SEO) for 31.52 percent, and 15 undefined for 16.30 percent. Hubei Province was classified serologically as a mixed prevalent area with Type I as its major component, but all serotypes in different sub areas have their own features and those in the old epidemic foci were more complex. It indicated that it was better to use a bivalent HFRS virus vaccine, or a single valent vaccine consistent with local serotype. Serotyping of local HFRS conformed basically to that of epidemiological classification. Local HFRS should be serotyped periodically due to continuous changes in types of foci. Attention to reactions of vaccine immunization should be paid during observation of the effectiveness of the vaccine.