镉对骨形态发生蛋白诱导软骨和骨形成的影响

Influence of Cadmium on Cartilage and Bone Formation Induced by Bone Morphogenetic Protein

为研究镉对骨组织的直接作用，利用骨形态发生蛋白建立诱导性异位成骨模型，研究镉对软骨和骨形成的影响，采用３５Ｓ、４０Ｃａ掺入率及碱性磷酸酶（ＡＬＰ）和酸性磷酸酶（ＡＣＰ）活性四项指标反映软骨和骨的形成情况。结果表明：镉对软骨形成过程中的３５Ｓ掺入率无明显影响，但能使骨形成过程中４０Ｃａ掺入率下降，镉能抑制ＡＬＰ活性，但对ＡＣＰ活性无明显作用。由此可见，镉对软骨形成无明显影响，但对软骨内成骨却有明显抑制作用，镉能抑制成骨细胞功能和钙盐沉积。

An induced osteogenetic model was established using bone morphogenetic protein (BMP) to study direct effect of cadmium on bone tissue, cartilage and bone formation. Four indicators, i. e. incorporation of 35 S and 40 Ca, and activities of alkaline phosphatase (ALP), acidic phosphatase (ACP), were used to reflect cartilage and bone formation. Results showed that no obvious effect of cadmium on incorporation of 35 S was found, but cadmium could lower incorporation of 40 Ca, inhibit activities of ALP and have no obvious effect on activity of ACP in osteogenesis. Therefore, it is concluded that cadmium has no obvious influence on cartilage formation, but has obvious inhibitory effect on endochondral bone formation, and cadmium can inhibit the function of osteoblast and calcium salt deposit.