Skp2蛋白在口腔鳞癌中的表达及其与c-myc、p27蛋白表达的关系

Expression of Skp2 protein in oral squamous cell carcinoma and its relationship with c-myc and p27 protein expression.

目的:研究Skp2、c-myc及p27蛋白在口腔鳞癌组织中的表达。方法:免疫组化检测Skp2、c-myc及p27蛋白在54例口腔鳞癌和15例正常口腔黏膜组织中的表达。结果:口腔鳞癌组织中Skp2、c-myc阳性表达率分别为35.19%(19/54)和38.89%(21/54),显著高于正常口腔黏膜组织6.67%(1/15)、6.67%(1/15)(P<0.05);阳性表p27达率为55.56%(30/54)显著低于正常口腔黏膜组织86.67%(13/15)(P<0.05);Skp2的表达与口腔鳞癌的临床分期、病理分级、淋巴结转移显著相关(P<0.05),与年龄、性别、肿瘤部位等因素无关(P>0.05);口腔鳞癌中Skp2与c-myc表达呈正相关(r=0.562,P<0.01);与p27蛋白的表达呈负相关(r=-0.532,P<0.01)。结论:Skp2蛋白过表达在口腔鳞癌的发生及发展中起重要作用;并可能与c-myc蛋白有协同作用,Skp2蛋白过表达与靶蛋白p27蛋白降解有关,联合检测Skp2、c-myc及p27蛋白的表达有助于综合评估口腔鳞癌的生物学行为。

Objective：To study the expression of Skp2 and c-myc,p27 protein in oral squamous cell carcinoma （OSCC）. Method：The expression of Skp2 and c-myc,p27was detected in 54cases of OSCC and 12cases of normal oral tissues （NOT） by immuno-histochemistry. Result：The positive rate of Skp2,c-myc stain was 35.19 % （19/54） and38.89% （21/54） in OSCC tissues, which was significantly higher than that in NOT of 6.67% （1/15） and 6.67% （1/15） （P〈 0.05） ; The p27 staining frequency in OSCC of 55.56% （30/54） was significantly lower than that in NOT of 86.67% （13/15） （P〈0.05） ; The expression level of Skp2 was closely related to cell differentiation, TNM stages and lymph node metastasis （P〈0.05）, There was no relationship between Skp2 expression and age or sex and tumor site （P〉0. 05）, the expression levels of Skp2 and c-myc protein were positively correlated in OSCC （r=0.562, P〈0.01）and the expression levels of Skp2 and P27 protein were inversely correlated in OSCC （r=-0.532,P〈0.01）. Conclusion： Over expression of Skp2 may play important roles in the development and progression of OSCC, and it may cooperate with c-myc protein： expression of Skp2 proteih reduced the protein level of p27： combined detection of the expression of Skp2,c-myc and p27 protein may be useful in evaluating the biological behavior of OSCC.