翻白草黄酮对糖尿病小鼠血清胰岛素和胰岛素抗体的作用

The Effects of Flavonoids from Potentilla discolor Bunge. on the Levels of Insulin and Insulin Antibody in Serum in Diabetes Mice

目的观察翻白草黄酮对糖尿病小鼠血糖和血清胰岛素和胰岛素抵抗的影响。方法翻白草经溶剂提取,聚酰胺柱分离提取得黄酮提取物;用四氧嘧啶腹腔注射造模,将造模成功的小鼠随机分成为高剂量(216 mg.kg-1.d-1)、低剂量(108 mg.kg-1.d-1)、模型对照组。连续给药14 d后,观察翻白草黄酮对糖尿病小鼠体重、血糖、血清胰岛素和胰岛素抗体的影响。结果翻白草黄酮能很好地降低糖尿病小鼠的血糖,提高血清中胰岛素水平,但对血清胰岛素抗体影响不大。结论翻白草黄酮对糖尿病的治疗作用,可能是通过降低血糖水平和提高血清胰岛素水平来实现的。

Objective To study the effects of flavonoids from Potentilla discolor Bunge. （FPB） on the blood glucose ,the levels of insulin and insulin antibody in serum in diabetes mice and its mechamisms. Methods FPB were obtained from Potentilla discolor Bunge. through solvent extraction and polyamide columnar Chromatography . The model mice of alloxan - diabetes were randomly divided into these groups：high dose group（216 mg·kg%-1·d^-1） ,low dose group（ 108 mg·kg%-1·d^-1） and model group. Body weight, blood glucose, the levels insulin and insulin antibody in serum were measured after 14 days. Results FPB decreased blood glucose and enhanced level of insulin in serum, but level of insulin antibody in serum was not significantly changed. Conclusion FPB can decrease blood glucose by protecting the islet β cell in diabetes mice.