祖卡木颗粒中罂粟碱含量的反相高效液相色谱法测定

Determination of Papaverine in Zukamu Granules by RP-HPLC

目的采用反相高效液相色谱（RP-HPLC）法测定祖卡木颗粒中罂粟碱的含量。方法使用DIKMA C8色谱柱（250mm×4．6mm，5μm），甲醇-乙腈-1％三乙胺-1％乙酸铵（40：20：1：39）为流动相，流速1ml／min，检测波长为240nm。结果罂粟碱在0．0198～0．396μg范围内呈良好线性关系（r=0．9999），平均回收率为99．6％，RSD为1．5％。结论该法灵敏、准确，专属性强，重复性好，可以作为祖卡木颗粒的含量测定方法。

Objective To establish a method for determining papaverine in Zukamu Granules. Methods The separation was carried out on DIKMA Cs column （250 mm × 4.6 mm, 5μm）. The mobile phase was carbinol - acetonitrile - 1% triethylamine - 1% ammonium acetate （40： 20： 1： 39）with a flow rate of 1.0ml/min. The detection wavelength was set at 240 nm. Results Papaverine showed a good linear relationship in the range of 0.0198 - 0. 396μg（ r = 0. 999 9） and the average recovery was 99.6% , RSD wasl. 5%. Conclusion The method is sensitive,accurate, specific and repeatable. It can be used for quality control of Zukamu Granules.