有氧运动和大豆多肽对高脂饮食大鼠胰岛素抵抗形成和血脂代谢的干预作用

Intervention Effects of Aerobic Exercise and Soy Protein Hydrolyzate on the Informing of Insulin Resistance and Blood Lipid Metabolism in SD Rats Fed High Fat Diet

目的:探讨有氧运动和补充大豆多肽对高脂饮食大鼠胰岛素抵抗形成的干预作用及其机制。方法:在给SD大鼠喂饲高脂饮食的同时,进行10周的有氧运动锻炼或/和补充大豆多肽,实验结束时测定空腹血糖(FPG)、空腹胰岛素(FINS)、血清甘油三酯(TG)、胆固醇(TC)和游离脂肪酸(FFA)等指标,并计算胰岛素敏感性指数(ISI)、稳态模型-IR指数(HOMA-IR)。结果:1)10周的高脂饮食可使大鼠FPG、FINS、HOMA-IR指数显著升高,而ISI指数显著降低;2)在高脂饮食诱导大鼠IR形成的同时,进行10周60 min的有氧运动锻炼或补充SPH,可以显著降低高脂饮食大鼠FPG、FINS、HOMA-IR指数,提高ISI指数;与此同时,运动联合补充SPH可使高脂饮食大鼠FINS、HOMA-IR指数进一步降低,ISI进一步升高,但无显著性差异;3)HF组大鼠血清TG、TC和FFA显著高于对照组,运动训练或补充SPH可使高脂饮食大鼠血清TG、TC和FFA含量显著降低,且具有显著的交互作用。结论:1)10周的高脂饮食可以诱导大鼠IR的形成;2)运动训练或补充SPH可以显著地预防高脂饮食大鼠IR的形成,而且,运动锻炼联合补充SPH在一定程度上对预防高脂饮食大鼠IR的形成具有促进作用。3)高脂饮食诱导大鼠IR的形成机制与高脂饮食大鼠血脂代谢紊乱有关;而运动训练或补充SPH可以改善血脂代谢来预防IR的形成。

The research was conducted in order to explore intervention effects of aerobic exercise and soy protein hydrolyzate （SPH） on the informing of insulin resistance （IR） in SD rats fed high fat diet and its mechanisms. While the SD rats were fed high fat diet to form IR, aerobic exercise training was conducted and SPH was supplemented for ten weeks. The fasting plasma glucose （FPG）, fasting plasma insulin （FINS）, the serum triglyceride （TG）, total cholesterol （TC） and free fatty acid （FFA）were determined at the end of the experiment. Insulin sensitivity index （ISI） and homeostasis model assessment-IR index （HOMA-IR） were calculated. Results are： 1） ten-week long high fat diets might cause the remarkable increase of FPG, FINS and HOMA-IR, but obvious decrease of ISI; 2） sixty-minute aerobic training and supplemented SPH for ten weeks might reduce FPG, FINS, and HOMA-IR but obviously enhance the ISI in rats fed high fat diet. Exercise and supplemented SPH might cause FPG, FINS, and HOMA-IR in the rats fed high fat diet rats to reduce, and cause ISI to increase. However, there were no significant difference; 3） Serum TG, TC, and FFA concentrations in HF group were higher than those in control group. At the same time, exercise trainng or supplemented SPH might cause serum TG, TC and FFA content in high fat diet rats to reduce obviously. Conclusions are： 1） ten-week long high fat diets might induce the formation of IR in rats; 2） Aerobic training or supplement of SPH for ten weeks might prevent the formation of IR in the rats fed high fat diet. Aerobic exercise united supplement SPH had the promoter action to prevent the formation of IR in the rats fed high fat diet to a certain extent; 3） The mechanism of the high fat diet induced the formation of IR in rats, and was related with the disorder of blood lipid metabolism in the rats fed high diet, while aerobic training or supplemented SPI might prevent the formation of IR through improving blood lipid metabolism.