癫痫患者抗脑抗体及脑组织中HLA—Ⅱ类抗原的表达

Serum anti-encephalic antibodies and human leukocyte antigen class 1 antigen in brain from patients with epilepsy

目的:为获得癫痫患者免疫学异常的证据,本实验检测了患者血清中抗脑抗体(Anti-encephalic antibodies,AEAb)及脑组织人类白细胞抗原Ⅱ类抗原(Human leukocyte antigen class Ⅱantigen,HLA class Ⅱ antigen,HLA-Ⅱ类抗原),并与正常对照组比较。对象及方法:1.用ELISA方法测定37例癫痫患者血清抗脑自身抗体;2.借助免疫组织化学方法观察了HLA—Ⅱ类抗原在脑组织中表达与分布。结果:1.癫痫患者血清抗脑抗体高于正常对照;2.癫痫患者脑组织中星形胶质细胞和小胶质细胞异常表达HLA—Ⅱ类抗原。讨论:本实验证实癫痫患者存在一定程度的自身免疫应答异常。脑组织胶质细胞表面HLA—Ⅱ类抗原表达异常可能通过多种机制参与癫痫发病。

Background and Purpose: In order to attain evidence of immunology anomaly in patients with epilepsy, anti-encephalic antibodies (AEAb) in serum from epileptics determined. We also examined about human leukocyte antigen class Ⅱ antigen (HLA class Ⅱ antigen) in brain. Method: 1. AEAb was examined in 37 patient by ELISA; 2. Immunohistochemial techniques were used for the expression and distribution of HLA classed Ⅱ antigen in brain. Results; 1. The positive rate and the average levels of AEAb were significantly higher in epileptics than those in healthy controls and patients with encephalitis; 2. HLA class Ⅱ antigen was expressed in abnormally high densities on astrocytes and microglicals in epileptics as compared with controls. Conclusion: These data suggested that autoimmunity may be one of the factors predisposing to epilepsy. Disorder on HLA class Ⅱ antigen expresion on neuroglias may involved in the pathogensis of epilepsy.