摘要
目的探讨单纯疱疹病毒胸苷激酶（HSV-TK）基因导人T细胞克隆治疗溃疡性结肠炎（UC）大鼠的作用。方法将HSV-TK基因导人T细胞克隆回输至27只UC大鼠，观察刺激指数（SI）、CD4^＋、CD8^＋及白细胞介素（IL）-13和IL-4水平的变化，并比较治疗前后结肠病变的情况。结果UC大鼠结肠壁病变于tk^＋细胞克隆回输治疗2-3d出现炎性反应吸收改变，7-10d后结肠炎性反应基本消失；回输前SI值为7．39±1．24，回输后为2．67±0．87（P〈0．05），CD4^＋、CD8^＋水平、IL-13和IL-4水平明显下降，差异有统计学意义（P〈0．05）。结论T细胞克隆疫苗诱导和tk基因导入后杀伤效应的双重作用导致结肠自身抗原特异性免疫耐受的最终形成，HSV-TK自杀基因导入T细胞克隆回输方案是UC基因治疗研究和靶点设计的有效手段。
Objective To investigate the effect of herpes simplex virus-thymidine kinase （HSV- TK） gene transduced into T cell clone on the treatment of ulcerative colitis （UC） in rat. Methods The T cell clone transduced with HSV-tk was infused into 27 rats with UC. Changes of stimulation index （SI）, CD4^＋, CD8^＋, IL-13, IL-4 were detected, and pathological changes before and after infusion was compared. Results In the second and third day after tk^＋ T clone infusion, the inflammation of colon was assimilated. Two weeks later, the colon began to renovate and mend. The average SI was 7.39±1.24 before infusion, and 2.67±0.87 after infusion （P〈0.05）. Peripheral blood levels of CD4^＋, CD8^＋ or IL-13 and IL-4 in therapeutic group were significantly decreased as compared to control group （P〈0.05）. Conclusion HSV-tk gene transduced into T lymphocyte clone and infused back is effective for UC gene therapy and target design in rat.
出处
《中华胃肠外科杂志》
CAS
2008年第1期72-75,共4页
Chinese Journal of Gastrointestinal Surgery
基金
国家自然科学基金资助项目（39970335）
广东省自然科学基金资助项目（970055）
