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成年大鼠小脑Purkinje神经元不表达神经元特异性的钾离子氯离子共转运体2 被引量：1

Absent expression of neuronal-specific K-Cl cotransporter 2 in adult rat purkinje neurons
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摘要 目的:观察神经元特异性标记物钾离子氯离子共转运体2(KCC2)在成年大鼠小脑皮质的表达。方法:成年SD大鼠,4%多聚甲醛灌注固定,取脑,冷冻切片,KCC2和小脑Purkinje神经元标记物Calbindin-D28K在小脑的免疫荧光双标,激光共聚焦显微镜观察。结果:KCC2在颗粒细胞层神经元有丰富的表达,但在Purkinje神经元表达很弱。结论:神经特异性的KCC2可能不是成年大鼠的Purkinje神经元的离子型γ-氨基丁酸受体发挥抑制性作用的主要因素。 Objective： To observe the neuronal specific expression of K-Cl cotranporter 2 （KCC2） in adult rat Purkinje neurons. Methods： The adult SD rats were deprived by 4% paraformaldehyde and the brains were taken out for iced section. The expressions of KCC2 and Calbindin-D28 were detected by immunofluorescence and the photos were observed with a confocal microscope. Results： The expression of KCC2 was absent from Purkinje neurons of the adult rat cerebellum. Conclusion： The neuronal specific KCC2 may not contribute to inotropic GABA receptors mediated-inhibition in Purkinje neurons in the adult rat cerebellum.
作者 王德广 张红红 张静 陈幽婷
机构地区 江苏省麻醉学研究所 徐州医学院神经生物学教研室 徐州医学院解剖学教研室
出处 《解剖学杂志》 CAS CSCD 北大核心 2008年第1期34-36,共3页 Chinese Journal of Anatomy
基金 江苏省教育厅资助课题(06KJD310188) 江苏省麻醉医学研究所开放课题(卫WK200601)
关键词 小脑 CALBINDIN-D28K 钾离子氯离子共转运体2 Purkinje神经元 cerebellum calbindin-D28K K-Cl cotranporter 2 Purkinje neuron
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
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参考文献8

  • 1Payne J A. Functional characterization of the neuronal-specific K-Cl cotransporter: implications for [K^+] regulation [J]. Am J Physiol, 1997,273(5 Pt 1):C1516-C1525.
  • 2Rivera C, Voipio J, Payne J A, et al. The K^+/Cl^-co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation [J]. Nature, 1999,397(6716):251-255.
  • 3Kanaka C, Ohno K. Okabe A, et al. The differential expression patterns of messenger RNAs encoding K-Cl cotransporters (KCC1,2) and Na-K-2Cl cotransporter (NKCC1) in the rat nervous system [J]. Neuroscience, 2001,104(4):933-946.
  • 4Wang D G, Gong N, Luo B, et al. Absence of GABA type A signaling in adult medial habenular neurons [J]. Neuroscience, 2006, 141(1):133-141.
  • 5Chen G, Racay P, Bichet S, et al. Deficiency in parvalbumin, but not in calbindin D-28k upregulates mitochondrial volume and decreases smooth endoplasmic reticulum surface selectively in a peripheral, subplasmalemmal region in the soma of Purkinje cells [J]. Neuroscience, 2006,142 (1) : 97-105.
  • 6Batini C. Cerebellar localization and colocalization of GABA and calcium binding protein-D28K [J]. Arch Ital Biol, 1990,128(2-4) : 127-149.
  • 7Fritschy J M, Panzanelli P, Kralic J E, et al. Differential dependence of axo-dendritic and axo-somatic GABAergic synapses on GABAA receptors containing the alphal subunit in Purkinje cells [J]. J Neurosci, 2006,26(12) :3245-355.
  • 8Takayama C, Inoue Y. Developmental localization of potassium chloride co-transporter 2 (KCC2) in the Purkinje cells of embryonic mouse cerebellum [J]. Neurosci Res, 2007,57(2):322-325.

同被引文献11

  • 1Williams JR, Sharp JW, Kumari VG, et al. The neuron - specific K- Cl cotransporter, KCC2. Antibody development and initial characterization of the protein [ J ]. J Biol Chem, 1999, 274(18) : 12656 - 12664.
  • 2Li H, Khirug S, Cai C, et al. KCC2 Interacts with the dendritic cytoskeleton to promote spine development [ J ]. Neuron, 2007, 56(6) : 1019 - 1033.
  • 3Reynolds A, Brustein E, Liao M, et al. Neurogenie role of the depolarizing chloride gradient revealed by global overexpression of KCC2 from the onset of development [ J ]. J Neurosci, 2008, 28 (7) : 1588 -1597.
  • 4Payne JA. Functional characterization of the neuronal - specific K - Cl cotransporter : implications for [ K^+ ] o regulation [ J ]. Am J Physiol, 1997, 273(5 Pt 1): C1516-C1525.
  • 5Wang DG, Gong N, Luo B, et al. Absence of GABA type A signaling in adult medial habenular neurons [ J ]. Neuroscience, 2006, 141(1): 133-141.
  • 6Ben - Ari Y. Excitatory actions of gaba during development: the nature of the nurture [J]. Nat Rev Neurosci, 2002, 3(9): 728 - 739.
  • 7Rivera C, Voipio J, Payne JA, et al. The K^+/Cl^- co -transporter KCC2 renders GABA hyperpolarizing during neuronal maturation [J]. Nature, 1999, 397(6716): 251 -255.
  • 8Mullen R J, Buck CR, Smith AM. NeuN, a neuronal specific nuclear protein in vertebrates [ J ]. Development, 1992,116 ( 1 ) : 201 - 211.
  • 9Wolf HK, Buslei R, Schmidt - Kastner R, et al. NeuN: a useful neuronal marker for diagnostic histopathology [ J ]. J Histochem Cytochem, 1996, 44(10) : 1167 -1171.
  • 10Park JB, Skalska S, Son S, et al. Dual GABAA receptor - mediated inhibition in rat presympathetic paraventricular nucleus neuroils [J]. J Physiol, 2007, 582(Pt 2) : 539-551.

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解剖学杂志
2008年 第1期

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