吗啡依赖戒断焦虑大鼠海马突触结构的可塑性

Synaptic plasticity of hippocampal neurons in anxious rats with morphine-withdrawal

目的:通过对吗啡依赖戒断后焦虑模型大鼠海马突触形态结构可塑性的观察,探讨其焦虑情绪的发生机制。方法:采用剂量递增法建立大鼠吗啡依赖戒断后焦虑模型。取海马CA1、CA3区组织进行透射电镜观察和体视学定量分析。结果:焦虑模型组大鼠海马CA1、CA3区均可见突触密集、数量多,但体积比较小。CA1区突触数密度、面密度均较对照组显著增高,而突触连接带平均面积显著减小;CA3区突触数密度、面密度较对照组同样明显增高,而突触连接带平均面积明显减小。结论:突触形态结构的可塑性变化可能参与了吗啡依赖戒断后焦虑情绪的发生。

Objective： To explore the mechanism of anxiety by observing synaptic plasticity on hippocampus in rat with morphine-withdrawal. Methods： Gradually increasing dosage was used to establish the model of morphine-dependence before the anxious model of morphine-withdrawal was finished. Tissues of CA1 and CA3 regions in hippocampus were observed through an electron microscope, and further quantitatively analyzed by stereological ways. Results： Dense and numerous synapses in CA1 and CA3 regions of hippocampus appeared in anxious rats, though they seemed smaller. By control, numerical density and surface density of CA1 were notably increased, whereas the mean profile area of synapse was decreased. Numerical density and surface density of CA3 were significantly increased; however, the mean profile area of synapse was decreased. Conclusion ： Variable configuration of synapse may be related to anxiety symptom in morphine-dependent rodents.